Abstract
Cells of the oligodendroglial lineage express Ca2+-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate-preferring glutamate receptors (AMPA-GluR) during development. Prolonged activation of their AMPA-GluR causes Ca2+ overload, resulting in excitotoxic death. Prior studies have shown that oligodendroglial progenitors and immature oligodendrocytes are susceptible to excitotoxicity, whereas mature oligodendrocytes are resistant. An unresolved issue has been why Ca2+-permeability of AMPA-GluR varies so markedly with oligodendroglial development, although the level of expression of edited GluR2, an AMPA-GluR subunit which blocks Ca2+ entry, is relatively constant. To address this question, we performed Ca2+ imaging, molecular and electrophysiological analyses using purified cultures of the rat oligodendroglial lineage. We demonstrate that transient up-regulation of expression of GluR3 and GluR4 subunits in oligodendroglial progenitors and immature oligodendrocytes results in the assembly by these cells, but not by oligodendroglial pre-progenitors or mature oligodendrocytes, of a population of AMPA-GluR which lack GluR2. This stage-specific up-regulation of edited GluR2-free, and hence Ca2+-permeable, AMPA-GluR explains the selective susceptibility to excitotoxicity of cells at these stages of oligodendroglial differentiation, and is likely to be important to these cells in the trans-synaptic Ca2+-signaling from glutamatergic neurons, which occurs in hippocampus in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 390-402 |
| Number of pages | 13 |
| Journal | Journal of Neurochemistry |
| Volume | 81 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- AMPA receptor
- Calcium
- Electrophysiology
- Fura-2 microfluorometry
- Oligodendroglial lineage
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
AMPA glutamate receptor-mediated calcium signaling is transiently enhanced during development of oligodendrocytes. / Ito, Takayuki; Beesley, Jacqueline; Itoh, Aki; Cohen, Akiva S.; Kavanaugh, Bryan; Coulter, Douglas A.; Grinspan, Judith B.; Pleasure, David E.
In: Journal of Neurochemistry, Vol. 81, No. 2, 2002, p. 390-402.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - AMPA glutamate receptor-mediated calcium signaling is transiently enhanced during development of oligodendrocytes
AU - Ito, Takayuki
AU - Beesley, Jacqueline
AU - Itoh, Aki
AU - Cohen, Akiva S.
AU - Kavanaugh, Bryan
AU - Coulter, Douglas A.
AU - Grinspan, Judith B.
AU - Pleasure, David E
PY - 2002
Y1 - 2002
N2 - Cells of the oligodendroglial lineage express Ca2+-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate-preferring glutamate receptors (AMPA-GluR) during development. Prolonged activation of their AMPA-GluR causes Ca2+ overload, resulting in excitotoxic death. Prior studies have shown that oligodendroglial progenitors and immature oligodendrocytes are susceptible to excitotoxicity, whereas mature oligodendrocytes are resistant. An unresolved issue has been why Ca2+-permeability of AMPA-GluR varies so markedly with oligodendroglial development, although the level of expression of edited GluR2, an AMPA-GluR subunit which blocks Ca2+ entry, is relatively constant. To address this question, we performed Ca2+ imaging, molecular and electrophysiological analyses using purified cultures of the rat oligodendroglial lineage. We demonstrate that transient up-regulation of expression of GluR3 and GluR4 subunits in oligodendroglial progenitors and immature oligodendrocytes results in the assembly by these cells, but not by oligodendroglial pre-progenitors or mature oligodendrocytes, of a population of AMPA-GluR which lack GluR2. This stage-specific up-regulation of edited GluR2-free, and hence Ca2+-permeable, AMPA-GluR explains the selective susceptibility to excitotoxicity of cells at these stages of oligodendroglial differentiation, and is likely to be important to these cells in the trans-synaptic Ca2+-signaling from glutamatergic neurons, which occurs in hippocampus in vivo.
AB - Cells of the oligodendroglial lineage express Ca2+-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate-preferring glutamate receptors (AMPA-GluR) during development. Prolonged activation of their AMPA-GluR causes Ca2+ overload, resulting in excitotoxic death. Prior studies have shown that oligodendroglial progenitors and immature oligodendrocytes are susceptible to excitotoxicity, whereas mature oligodendrocytes are resistant. An unresolved issue has been why Ca2+-permeability of AMPA-GluR varies so markedly with oligodendroglial development, although the level of expression of edited GluR2, an AMPA-GluR subunit which blocks Ca2+ entry, is relatively constant. To address this question, we performed Ca2+ imaging, molecular and electrophysiological analyses using purified cultures of the rat oligodendroglial lineage. We demonstrate that transient up-regulation of expression of GluR3 and GluR4 subunits in oligodendroglial progenitors and immature oligodendrocytes results in the assembly by these cells, but not by oligodendroglial pre-progenitors or mature oligodendrocytes, of a population of AMPA-GluR which lack GluR2. This stage-specific up-regulation of edited GluR2-free, and hence Ca2+-permeable, AMPA-GluR explains the selective susceptibility to excitotoxicity of cells at these stages of oligodendroglial differentiation, and is likely to be important to these cells in the trans-synaptic Ca2+-signaling from glutamatergic neurons, which occurs in hippocampus in vivo.
KW - AMPA receptor
KW - Calcium
KW - Electrophysiology
KW - Fura-2 microfluorometry
KW - Oligodendroglial lineage
UR - http://www.scopus.com/inward/record.url?scp=0036312570&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036312570&partnerID=8YFLogxK
U2 - 10.1046/j.1471-4159.2002.00866.x
DO - 10.1046/j.1471-4159.2002.00866.x
M3 - Article
C2 - 12064486
AN - SCOPUS:0036312570
VL - 81
SP - 390
EP - 402
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 2
ER -