Amount of calcium in the sarcoplasmic reticulum: Influence on excitation-contraction coupling in heart muscle

Luis Fernando Santana, Ana M. Gómez, Evangelina G. Kranias, W. Jonathan Lederer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Real-time imaging of the concentration of intracellular calcium ([Ca2+](i)) has been carried out in heart cells using confocal imaging and patch-clamp techniques. Here we review recent investigations that used genetically engineered mice that lack phospholamban (PL knockout) to investigate the mechanisms of excitation-contraction (EC) coupling in heart. The heart cells from PL knockout (KO) mice exhibit [Ca2+](i) transients that are larger than normal. Similarly, the elementary units of EC coupling, called Ca2+ sparks, were found to be more frequent in PL KO heart cells than normal heart cells. This finding is consistent with the findings that cell-wide [Ca2+](i) transients arise as Ca2+ sparks sum during the EC coupling process. Finally, it was determined that the amount of Ca2+ within the sarcoplasmic reticulum (SR) was greater in the PL KO cardiac myocytes than in controls. Analysis of the results indicate that the larger [Ca2+](i) transients and the more frequent Ca2+ sparks are due to the greater amount of Ca2+ within the SR of PL KO myocytes, as the Ca2+ current (I(Ca)), which triggers the Ca2+ sparks and the [Ca2+](i) transient, was the same in control and PL KO heart cells. We conclude that the amount of Ca2+ released from the SR per unit of triggering I(Ca) increases as the SR Ca2+ content is augmented. Regulation of SR Ca2+ content is thus a means by which cardiac contractility is regulated.

Original languageEnglish (US)
Pages (from-to)44-49
Number of pages6
JournalHeart and Vessels
Volume12
Issue numberSUPPL. 12
StatePublished - 1997
Externally publishedYes

Fingerprint

Excitation Contraction Coupling
Sarcoplasmic Reticulum
Myocardium
Calcium
Patch-Clamp Techniques
Cardiac Myocytes
Knockout Mice
Muscle Cells

Keywords

  • Calcium
  • Confocal
  • Contraction
  • Heart
  • Phospholamban

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Amount of calcium in the sarcoplasmic reticulum : Influence on excitation-contraction coupling in heart muscle. / Santana, Luis Fernando; Gómez, Ana M.; Kranias, Evangelina G.; Jonathan Lederer, W.

In: Heart and Vessels, Vol. 12, No. SUPPL. 12, 1997, p. 44-49.

Research output: Contribution to journalArticle

Santana, LF, Gómez, AM, Kranias, EG & Jonathan Lederer, W 1997, 'Amount of calcium in the sarcoplasmic reticulum: Influence on excitation-contraction coupling in heart muscle', Heart and Vessels, vol. 12, no. SUPPL. 12, pp. 44-49.
Santana, Luis Fernando ; Gómez, Ana M. ; Kranias, Evangelina G. ; Jonathan Lederer, W. / Amount of calcium in the sarcoplasmic reticulum : Influence on excitation-contraction coupling in heart muscle. In: Heart and Vessels. 1997 ; Vol. 12, No. SUPPL. 12. pp. 44-49.
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