Amino acid substitutions in the structural or nonstructural proteins of a vaccine strain of equine arteritis virus are associated with its attenuation

Jianqiang Zhang, Yun Young Go, Nigel J Maclachlan, Barry J. Meade, Peter J. Timoney, Udeni B R Balasuriya

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Comparative sequence analysis of a series of strains of equine arteritis virus (EAV) of defined virulence for horses, ranging from the horse-adapted virulent Bucyrus (VB) strain to a fully attenuated vaccine strain derived from it, identified 13 amino acid substitutions associated with attenuation. These include 4 substitutions in the replicase proteins and 9 in the structural proteins. Using reverse genetic techniques, these amino acid substitutions were introduced into a virulent infectious cDNA clone pEAVrVBS derived from the VB strain of EAV. Inoculation of horses with the recombinant viruses clearly demonstrated that changes in either the replicase (nsp1, nsp2 and nsp7) or structural proteins (GP2, GP4, GP5 and M) resulted in attenuation of the virulent VB strain. The recombinant virus with substitutions in the structural proteins was more attenuated than the recombinant virus with substitutions only in the replicase proteins.

Original languageEnglish (US)
Pages (from-to)355-362
Number of pages8
JournalVirology
Volume378
Issue number2
DOIs
StatePublished - Sep 1 2008

Keywords

  • Attenuation
  • Equine arteritis virus
  • Infectious clone
  • Reverse genetics
  • Structural and nonstructural proteins
  • Vaccine

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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