Amino Acid Sequences of Proteins from Leptospira Serovar pomona

Selmo F. Alves, Rance B Lefebvre, William Probert

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45 kDa. In situ digestion of 40 kDa molecular weight protein was accomplished using cyanogen bromide. The 32 kDa protein generated two fragments, one of 21 kDa and another of 10 kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40 kDa protein.

Original languageEnglish (US)
Pages (from-to)503-504
Number of pages2
JournalMemorias do Instituto Oswaldo Cruz
Volume95
Issue number4
StatePublished - Jul 2000

Keywords

  • Amino acid
  • Leptospira
  • Proteins
  • Sequence

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Virology
  • Infectious Diseases
  • Microbiology (medical)

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