Amino acid replacements in the Serratia marcescens haemolysin ShlA define sites involved in activation and secretion

Roland Schönherr, Renee M Tsolis, Tony Focareta, Volkmar Braun

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

The haemolysin of Serratia marcescens (ShlA) is translocated through the cytoplasmic membrane by the signal peptide-dependent export apparatus. Translocation across the outer membrane (secretion) is mediated by the ShlB protein. Only the secreted form of ShlA is haemolytic. ShlB also converts in vitro inactive ShlA (ShlA*), synthesized in the absence of ShlB, into the haemolytic form (a process termed activation). To define regions in ShlA involved in both processes, ShlA derivatives were isolated and tested for secretion and activation. Analysis of C-terminally truncated proteins (ShlA′) assigned the secretion signal to the amino-terminal 238 residues of ShlA. Trypsin cleavage of a secreted ShlA′ derivative yielded a 15kDa N-terminal fragment, by which a haemolytically inactive ShlA* protein could be activated in vitro. It is suggested that the haemolysin activation site is located in this N-terminal fragment. Replacement of asparagine-69 and asparagine-109 by isoleucine yielded inactive haemolysin derivatives. Both asparagine residues are part of two short sequence motifs, reading Ala-Asn-Pro-Asn, which are critical to both activation and secretion. These point mutants as well as N-terminal deletion derivatives which were not activated by ShlB were activated by adding a non-haemolytic N-terminal fragment synthesized in an ShlB+ strain (complementation). Apparently the activated N-terminal fragment substituted for the missing activation of the ShlA derivatives and directed them into the erythrocyte membrane, where they formed pores. It is concluded that activation is only required for initiation of pore formation, and that in vivo activation and secretion are tightly coupled processes. Complementation may also indicate that haemolysin oligomers form the pores.

Original languageEnglish (US)
Pages (from-to)1229-1237
Number of pages9
JournalMolecular Microbiology
Volume9
Issue number6
StatePublished - Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

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