Alzheimer's disease drug candidates stabilize A-β protein native structure by interacting with the hydrophobic core

Jie Li, Ruiwu Liu, Kit Lam, Lee-Way Jin, Yong Duan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Deposition of amyloid fibrils, consisting primarily of Aβ40 and Aβ42 peptides, in the extracellular space in the brain is a major characteristic of Alzheimer's disease (AD). We recently developed new (to our knowledge) drug candidates for AD that inhibit the fibril formation of Aβ peptides and eliminate their neurotoxicity. We performed all-atom molecular-dynamics simulations on the Aβ42 monomer at its α-helical conformation and a pentamer fibril fragment of Aβ42 peptide with or without LRL and fluorene series compounds to investigate the mechanism of inhibition. The results show that the active drug candidates, LRL22 (EC50 = 0.734 μM) and K162 (EC50 = 0.080 μM), stabilize hydrophobic core I of Aβ42 peptide (residues 17-21) to its α-helical conformation by interacting specifically in this region. The nonactive drug candidates, LRL27 (EC50 > 10 μM) and K182 (EC50 > 5 μM), have little to no similar effect. This explains the different behavior of the drug candidates in experiments. Of more importance, this phenomenon indicates that hydrophobic core I of the Aβ42 peptide plays a major mechanistic role in the formation of amyloid fibrils, and paves the way for the development of new drugs against AD.

Original languageEnglish (US)
Pages (from-to)1076-1082
Number of pages7
JournalBiophysical Journal
Volume100
Issue number4
DOIs
StatePublished - Feb 16 2011

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Alzheimer Disease
Pharmaceutical Preparations
Proteins
Amyloid
Peptide Fragments
Extracellular Space
Molecular Dynamics Simulation
Peptides
peptide A42
Brain

ASJC Scopus subject areas

  • Biophysics

Cite this

Alzheimer's disease drug candidates stabilize A-β protein native structure by interacting with the hydrophobic core. / Li, Jie; Liu, Ruiwu; Lam, Kit; Jin, Lee-Way; Duan, Yong.

In: Biophysical Journal, Vol. 100, No. 4, 16.02.2011, p. 1076-1082.

Research output: Contribution to journalArticle

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