Alveolar macrophage reaction to PM 2.5 of hazy day in vitro: Evaluation methods and mitochondrial screening to determine mechanisms of biological effect

Haiying Wei, Yunyun Zhang, Shanjuan Song, Kent E Pinkerton, Hong Geng, Chul Un Ro

Research output: Contribution to journalArticle

Abstract

Hazy weather in China has recently become a major public health concern due to high levels of atmospheric fine particulate matter (PM 2.5 ) with a large amount of polycyclic aromatic hydrocarbon (PAHs). In this study, the mass concentration of PAHs in hazy PM 2.5 in urban Taiyuan city, China was determined and toxicities of different dosage of the hazy PM 2.5 on rat alveolar macrophages (AMs) were examined. It was found that the hazy PM 2.5 , bounded with many species of PAHs (CHR, BbF, BaP, BaA, and etc.), significantly increased cellular malondialdehyde (MDA) content followed by the decreasing in superoxide (SOD) and glutathione peroxidase (GPx) in AMs. They induced mitochondrial changes in ultrastructure as evidenced by mitochondrial swelling and cristae disorganization, and a dose-dependent decrease in mitochondrial profile density. Also, the mRNA expression levels of mitochondrial fusion-related genes were modified. The Mfn1 and Mfn2 which are essential for mitochondrial fusion increased significantly in hazy PM 2.5 -treated group compared to the control in a dose-dependent manner, OPA1 was significantly increased at the highest PM 2.5 dose delivered. These findings suggested that exposure to hazy PM 2.5 could activate oxidative stress pathways in AMs, resulting in abnormal mitochondrial morphology and fusion/fission frequency. Possibly, the toxic effects were mostly attributed to the high burden of varied PAHs in hazy PM 2.5 .

Original languageEnglish (US)
Pages (from-to)566-573
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume174
DOIs
StatePublished - Jun 15 2019

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Polycyclic Aromatic Hydrocarbons
Alveolar Macrophages
Mitochondrial Dynamics
Polycyclic aromatic hydrocarbons
Screening
Fusion reactions
China
Mitochondrial Swelling
Oxidative stress
Particulate Matter
Poisons
Weather
Public health
Glutathione Peroxidase
Malondialdehyde
Superoxides
Toxicity
Swelling
Rats
Oxidative Stress

Keywords

  • Alveolar macrophage
  • Fusion/fission
  • Hazy
  • Mitochondria
  • PM

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Alveolar macrophage reaction to PM 2.5 of hazy day in vitro : Evaluation methods and mitochondrial screening to determine mechanisms of biological effect. / Wei, Haiying; Zhang, Yunyun; Song, Shanjuan; Pinkerton, Kent E; Geng, Hong; Ro, Chul Un.

In: Ecotoxicology and Environmental Safety, Vol. 174, 15.06.2019, p. 566-573.

Research output: Contribution to journalArticle

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