Abstract
The present study was conducted to characterize the possible interaction of Al3+ and Fe2+ with synthetic melanin in the potentiation of lipid peroxidation in liposomes and rat caudate-putamen homogenates. Al3+ stimulated melanin-initiated lipid peroxidation as measured by the production of 2-thiobarbituric acid-reactive substances (TBARS) and conjugated dienes. The effect of Al3+ was dependent on melanin (10-100 μg/ml) and Al3+ (2.5-250 μM) concentrations and no synergism between Fe2+ and Al3+ was observed. The prooxidant effect of Al3+ was partially inhibited by superoxide dismutase indicating the involvement of Q2/·-, Ga3+ and Be2+ which can increase NADH oxidation in the presence of Q2/·-, also were shown to stimulate melanin-initiated TBARS production. Based on the effect of Al3+ and other non redox metals, we suggest that Al3+ does not act through either the induction of melanin free radicals, or the induction of changes in membrane physical properties. Results show that Al3+ enhances melanin-initiated lipid peroxidation in part through an interaction with O2/·- generated from the autoxidation of melanin. We speculate that Al3+ contributes to neuromelanin-mediated oxidative damage in dopaminergic neurons and subsequent neuronal degeneration and death in Parkinson's disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1001-1008 |
| Number of pages | 8 |
| Journal | Neurochemical Research |
| Volume | 24 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- Aluminum
- Free radicals
- Lipid peroxidation
- Melanin
- Neuromelanin
- Parkinson
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry
Cite this
Aluminum enhances melanin-induced lipid peroxidation. / Meglio, Leonardo; Oteiza, Patricia I.
In: Neurochemical Research, Vol. 24, No. 8, 1999, p. 1001-1008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aluminum enhances melanin-induced lipid peroxidation
AU - Meglio, Leonardo
AU - Oteiza, Patricia I.
PY - 1999
Y1 - 1999
N2 - The present study was conducted to characterize the possible interaction of Al3+ and Fe2+ with synthetic melanin in the potentiation of lipid peroxidation in liposomes and rat caudate-putamen homogenates. Al3+ stimulated melanin-initiated lipid peroxidation as measured by the production of 2-thiobarbituric acid-reactive substances (TBARS) and conjugated dienes. The effect of Al3+ was dependent on melanin (10-100 μg/ml) and Al3+ (2.5-250 μM) concentrations and no synergism between Fe2+ and Al3+ was observed. The prooxidant effect of Al3+ was partially inhibited by superoxide dismutase indicating the involvement of Q2/·-, Ga3+ and Be2+ which can increase NADH oxidation in the presence of Q2/·-, also were shown to stimulate melanin-initiated TBARS production. Based on the effect of Al3+ and other non redox metals, we suggest that Al3+ does not act through either the induction of melanin free radicals, or the induction of changes in membrane physical properties. Results show that Al3+ enhances melanin-initiated lipid peroxidation in part through an interaction with O2/·- generated from the autoxidation of melanin. We speculate that Al3+ contributes to neuromelanin-mediated oxidative damage in dopaminergic neurons and subsequent neuronal degeneration and death in Parkinson's disease.
AB - The present study was conducted to characterize the possible interaction of Al3+ and Fe2+ with synthetic melanin in the potentiation of lipid peroxidation in liposomes and rat caudate-putamen homogenates. Al3+ stimulated melanin-initiated lipid peroxidation as measured by the production of 2-thiobarbituric acid-reactive substances (TBARS) and conjugated dienes. The effect of Al3+ was dependent on melanin (10-100 μg/ml) and Al3+ (2.5-250 μM) concentrations and no synergism between Fe2+ and Al3+ was observed. The prooxidant effect of Al3+ was partially inhibited by superoxide dismutase indicating the involvement of Q2/·-, Ga3+ and Be2+ which can increase NADH oxidation in the presence of Q2/·-, also were shown to stimulate melanin-initiated TBARS production. Based on the effect of Al3+ and other non redox metals, we suggest that Al3+ does not act through either the induction of melanin free radicals, or the induction of changes in membrane physical properties. Results show that Al3+ enhances melanin-initiated lipid peroxidation in part through an interaction with O2/·- generated from the autoxidation of melanin. We speculate that Al3+ contributes to neuromelanin-mediated oxidative damage in dopaminergic neurons and subsequent neuronal degeneration and death in Parkinson's disease.
KW - Aluminum
KW - Free radicals
KW - Lipid peroxidation
KW - Melanin
KW - Neuromelanin
KW - Parkinson
UR - http://www.scopus.com/inward/record.url?scp=0032818101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032818101&partnerID=8YFLogxK
U2 - 10.1023/A:1021000709082
DO - 10.1023/A:1021000709082
M3 - Article
C2 - 10478939
AN - SCOPUS:0032818101
VL - 24
SP - 1001
EP - 1008
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 8
ER -