Aluminum alters iron and manganese uptake and regulation of surface transferrin receptors in primary rat oligodendrocyte cultures

Mari S. Golub, Bin Han, Carl L Keen

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Transferrin (Tf) is a major transport protein for both iron (Fe) and aluminum (Al), as well as manganese (Mn) and it can mediate cellular uptake of these elements via cell surface Tf receptors. To study the effect of Al-Tf on Tf receptor regulation, primary oligodendrocyte cultures were prepared from cortices of newborn rats. The effects of Al-Tf on 54Mn and 59Fe uptake were compared to those of apo-, Fe-, or Mn-Tf (1.25 μM). To examine changes in cell surface Tf binding capacity, preincubation (4 h, 37°C) was performed with apo-, Al- or Fe-Tf and homologous receptor binding studies were subsequently conducted with 125I-Fe-Tf at 4°C. Incubation with AL-Tf, but not with equimolar amounts of Al chloride or Al citrate, led to dose-related increases in cellular Al. Incubation with either Al- or Fe-Tf decreased 59Fe uptake, while incubation with either Al- or Mn-Tf decreased 54Mn uptake. Surface Tf receptor sites/cell were 1.05, 0.60 and 0.46 x 105 after incubations with equivalent amounts of apo-, Fe-, and Al-Tf respectively. The data suggest that Al-Tf down-regulates surface Tf receptors on oligodendrocytes and can limit Fe and Mn uptake through this mechanism.

Original languageEnglish (US)
Pages (from-to)72-77
Number of pages6
JournalBrain Research
Volume719
Issue number1-2
DOIs
StatePublished - May 6 1996

Keywords

  • Aluminium
  • Iron
  • Manganese
  • Oligodendrocyte
  • Transferrin
  • Transferrin receptor

ASJC Scopus subject areas

  • Neuroscience(all)

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