Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure

Jean Jacques Mercadier, Anne Marie Lompré, Philippe Duc, Kenneth R. Boheler, Jean Bernard Fraysse, Claudine Wisnewsky, Paul D. Allen, Michel Komajda, Ketty Schwartz

Research output: Contribution to journalArticle

392 Citations (Scopus)

Abstract

A decrease in the myocardial level of the mRNA encoding the Ca2+-ATPase of the sarcoplasmic reticulum (SR) has been recently reported during experimental cardiac hypertrophy and failure. To determine if such a deficit occurs in human end-stage heart failure, we compared the SR Ca2+-ATPase mRNA levels in left (LV) and right ventricular (RV) specimens from 13 patients undergoing cardiac transplantation (6 idiopathic dilated cardiomyopathies; 4 coronary artery diseases with myocardial infarctions; 3 diverse etiologies) with control heart samples using a rat cardiac SR Ca2+-ATPase cDNA probe. We observed a marked decrease in the mRNA for the Ca2+-ATP-ase relative to both the 18S ribosomal RNA and the myosin heavy chain mRNA in LV specimens of patients with heart failure compared to controls (-48%, P < 0.01 and -47%, P < 0.05, respectively). The LV ratio of Ca2+-ATPase mRNA to 18S RNA positively correlated with cardiac index (P < 0.02). The RV ratio correlated negatively with systolic, diastolic and mean pulmonary arterial pressures (P < 0.02, P < 0.02, and P < 0.01, respectively). We suggest that a decrease of the SR Ca2+-ATPase mRNA in the myocardium plays an important role in alterations of Ca2+ movements and myocardial relaxation reported during human end-stage heart failure.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalJournal of Clinical Investigation
Volume85
Issue number1
StatePublished - Jan 1990
Externally publishedYes

Fingerprint

Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Heart Failure
Gene Expression
Messenger RNA
18S Ribosomal RNA
Myosin Heavy Chains
Dilated Cardiomyopathy
Cardiomegaly
Heart Transplantation
Myocardium
Arterial Pressure
Complementary DNA
Adenosine Triphosphate
RNA
Lung

Keywords

  • Gene expression
  • Heart failure
  • Human left ventricle
  • Myosin heavy chain mRNA
  • Sarcoplasmic reticulum Ca-ATPase mRNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mercadier, J. J., Lompré, A. M., Duc, P., Boheler, K. R., Fraysse, J. B., Wisnewsky, C., ... Schwartz, K. (1990). Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure. Journal of Clinical Investigation, 85(1), 305-309.

Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure. / Mercadier, Jean Jacques; Lompré, Anne Marie; Duc, Philippe; Boheler, Kenneth R.; Fraysse, Jean Bernard; Wisnewsky, Claudine; Allen, Paul D.; Komajda, Michel; Schwartz, Ketty.

In: Journal of Clinical Investigation, Vol. 85, No. 1, 01.1990, p. 305-309.

Research output: Contribution to journalArticle

Mercadier, JJ, Lompré, AM, Duc, P, Boheler, KR, Fraysse, JB, Wisnewsky, C, Allen, PD, Komajda, M & Schwartz, K 1990, 'Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure', Journal of Clinical Investigation, vol. 85, no. 1, pp. 305-309.
Mercadier JJ, Lompré AM, Duc P, Boheler KR, Fraysse JB, Wisnewsky C et al. Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure. Journal of Clinical Investigation. 1990 Jan;85(1):305-309.
Mercadier, Jean Jacques ; Lompré, Anne Marie ; Duc, Philippe ; Boheler, Kenneth R. ; Fraysse, Jean Bernard ; Wisnewsky, Claudine ; Allen, Paul D. ; Komajda, Michel ; Schwartz, Ketty. / Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure. In: Journal of Clinical Investigation. 1990 ; Vol. 85, No. 1. pp. 305-309.
@article{821a9b39889f421995044f57dc0ff026,
title = "Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure",
abstract = "A decrease in the myocardial level of the mRNA encoding the Ca2+-ATPase of the sarcoplasmic reticulum (SR) has been recently reported during experimental cardiac hypertrophy and failure. To determine if such a deficit occurs in human end-stage heart failure, we compared the SR Ca2+-ATPase mRNA levels in left (LV) and right ventricular (RV) specimens from 13 patients undergoing cardiac transplantation (6 idiopathic dilated cardiomyopathies; 4 coronary artery diseases with myocardial infarctions; 3 diverse etiologies) with control heart samples using a rat cardiac SR Ca2+-ATPase cDNA probe. We observed a marked decrease in the mRNA for the Ca2+-ATP-ase relative to both the 18S ribosomal RNA and the myosin heavy chain mRNA in LV specimens of patients with heart failure compared to controls (-48{\%}, P < 0.01 and -47{\%}, P < 0.05, respectively). The LV ratio of Ca2+-ATPase mRNA to 18S RNA positively correlated with cardiac index (P < 0.02). The RV ratio correlated negatively with systolic, diastolic and mean pulmonary arterial pressures (P < 0.02, P < 0.02, and P < 0.01, respectively). We suggest that a decrease of the SR Ca2+-ATPase mRNA in the myocardium plays an important role in alterations of Ca2+ movements and myocardial relaxation reported during human end-stage heart failure.",
keywords = "Gene expression, Heart failure, Human left ventricle, Myosin heavy chain mRNA, Sarcoplasmic reticulum Ca-ATPase mRNA",
author = "Mercadier, {Jean Jacques} and Lompr{\'e}, {Anne Marie} and Philippe Duc and Boheler, {Kenneth R.} and Fraysse, {Jean Bernard} and Claudine Wisnewsky and Allen, {Paul D.} and Michel Komajda and Ketty Schwartz",
year = "1990",
month = "1",
language = "English (US)",
volume = "85",
pages = "305--309",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "1",

}

TY - JOUR

T1 - Altered sarcoplasmic reticulum Ca2+-ATPase gene expression in the human ventricle during end-stage heart failure

AU - Mercadier, Jean Jacques

AU - Lompré, Anne Marie

AU - Duc, Philippe

AU - Boheler, Kenneth R.

AU - Fraysse, Jean Bernard

AU - Wisnewsky, Claudine

AU - Allen, Paul D.

AU - Komajda, Michel

AU - Schwartz, Ketty

PY - 1990/1

Y1 - 1990/1

N2 - A decrease in the myocardial level of the mRNA encoding the Ca2+-ATPase of the sarcoplasmic reticulum (SR) has been recently reported during experimental cardiac hypertrophy and failure. To determine if such a deficit occurs in human end-stage heart failure, we compared the SR Ca2+-ATPase mRNA levels in left (LV) and right ventricular (RV) specimens from 13 patients undergoing cardiac transplantation (6 idiopathic dilated cardiomyopathies; 4 coronary artery diseases with myocardial infarctions; 3 diverse etiologies) with control heart samples using a rat cardiac SR Ca2+-ATPase cDNA probe. We observed a marked decrease in the mRNA for the Ca2+-ATP-ase relative to both the 18S ribosomal RNA and the myosin heavy chain mRNA in LV specimens of patients with heart failure compared to controls (-48%, P < 0.01 and -47%, P < 0.05, respectively). The LV ratio of Ca2+-ATPase mRNA to 18S RNA positively correlated with cardiac index (P < 0.02). The RV ratio correlated negatively with systolic, diastolic and mean pulmonary arterial pressures (P < 0.02, P < 0.02, and P < 0.01, respectively). We suggest that a decrease of the SR Ca2+-ATPase mRNA in the myocardium plays an important role in alterations of Ca2+ movements and myocardial relaxation reported during human end-stage heart failure.

AB - A decrease in the myocardial level of the mRNA encoding the Ca2+-ATPase of the sarcoplasmic reticulum (SR) has been recently reported during experimental cardiac hypertrophy and failure. To determine if such a deficit occurs in human end-stage heart failure, we compared the SR Ca2+-ATPase mRNA levels in left (LV) and right ventricular (RV) specimens from 13 patients undergoing cardiac transplantation (6 idiopathic dilated cardiomyopathies; 4 coronary artery diseases with myocardial infarctions; 3 diverse etiologies) with control heart samples using a rat cardiac SR Ca2+-ATPase cDNA probe. We observed a marked decrease in the mRNA for the Ca2+-ATP-ase relative to both the 18S ribosomal RNA and the myosin heavy chain mRNA in LV specimens of patients with heart failure compared to controls (-48%, P < 0.01 and -47%, P < 0.05, respectively). The LV ratio of Ca2+-ATPase mRNA to 18S RNA positively correlated with cardiac index (P < 0.02). The RV ratio correlated negatively with systolic, diastolic and mean pulmonary arterial pressures (P < 0.02, P < 0.02, and P < 0.01, respectively). We suggest that a decrease of the SR Ca2+-ATPase mRNA in the myocardium plays an important role in alterations of Ca2+ movements and myocardial relaxation reported during human end-stage heart failure.

KW - Gene expression

KW - Heart failure

KW - Human left ventricle

KW - Myosin heavy chain mRNA

KW - Sarcoplasmic reticulum Ca-ATPase mRNA

UR - http://www.scopus.com/inward/record.url?scp=0025169633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025169633&partnerID=8YFLogxK

M3 - Article

C2 - 2136864

AN - SCOPUS:0025169633

VL - 85

SP - 305

EP - 309

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 1

ER -