Abstract
Background & Aims Salmonella enterica serovar Typhimurium is an enteropathogen that causes self-limiting diarrhea in healthy individuals, but poses a significant health threat to vulnerable populations. Our understanding of the pathogenesis of Salmonella-induced diarrhea has been hampered by the lack of a suitable mouse model. After a dose of oral kanamycin, Salmonella-infected congenic BALB/c.D2NrampG169 mice, which carry a wild-type Nramp1 gene, develop clear manifestations of diarrhea. We used this model to elucidate the pathophysiology of Salmonella-induced diarrhea. Methods BALB /c.D2 NrampG169 mice were treated with kanamycin and then infected with wild-type or mutant Salmonella by oral gavage. Colon tissues were isolated and Ussing chambers, quantitative polymerase chain reaction, immunoblot, and confocal microscopy analyses were used to study function and expression of ion transporters and cell proliferation. Results Studies with Ussing chambers demonstrated reduced basal and/or adenosine 3′,5′-cyclic monophosphate-mediated electrogenic ion transport in infected colonic tissues, attributable to changes in chloride or sodium transport, depending on the segment studied. The effects of infection were mediated, at least in part, by effector proteins secreted by the bacterial Salmonella pathogenicity island 1- and Salmonella pathogenicity island-2-encoded virulence systems. Infected tissue showed reduced expression of the chloride-bicarbonate exchanger down-regulated in adenoma in surface colonic epithelial cells. Cystic fibrosis transmembrane conductance regulator was internalized in colonic crypt epithelial cells without a change in overall expression levels. Confocal analyses, densitometry, and quantitative polymerase chain reaction revealed that expression of epithelial sodium channel β was reduced in distal colons of Salmonella-infected mice. The changes in transporter expression, localization, and/or function were accompanied by crypt hyperplasia in Salmonella-infected mice. Conclusions Salmonella infection induces diarrhea by altering expression and/or function of transporters that mediate water absorption in the colon, likely reflecting the fact that epithelial cells have less time to differentiate into surface cells when proliferation rates are increased by infection.
| Original language | English (US) |
|---|---|
| Journal | Gastroenterology |
| Volume | 145 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2013 |
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Keywords
- Colon
- DRA
- ENaC
- Salmonella
ASJC Scopus subject areas
- Gastroenterology
Cite this
Altered expression and localization of ion transporters contribute to diarrhea in mice with Salmonella-induced enteritis. / Marchelletta, Ronald R.; Gareau, Melanie; McCole, Declan F.; Okamoto, Sharon; Roel, Elise; Klinkenberg, Rachel; Guiney, Donald G.; Fierer, Joshua; Barrett, Kim E.
In: Gastroenterology, Vol. 145, No. 6, 12.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Altered expression and localization of ion transporters contribute to diarrhea in mice with Salmonella-induced enteritis
AU - Marchelletta, Ronald R.
AU - Gareau, Melanie
AU - McCole, Declan F.
AU - Okamoto, Sharon
AU - Roel, Elise
AU - Klinkenberg, Rachel
AU - Guiney, Donald G.
AU - Fierer, Joshua
AU - Barrett, Kim E.
PY - 2013/12
Y1 - 2013/12
N2 - Background & Aims Salmonella enterica serovar Typhimurium is an enteropathogen that causes self-limiting diarrhea in healthy individuals, but poses a significant health threat to vulnerable populations. Our understanding of the pathogenesis of Salmonella-induced diarrhea has been hampered by the lack of a suitable mouse model. After a dose of oral kanamycin, Salmonella-infected congenic BALB/c.D2NrampG169 mice, which carry a wild-type Nramp1 gene, develop clear manifestations of diarrhea. We used this model to elucidate the pathophysiology of Salmonella-induced diarrhea. Methods BALB /c.D2 NrampG169 mice were treated with kanamycin and then infected with wild-type or mutant Salmonella by oral gavage. Colon tissues were isolated and Ussing chambers, quantitative polymerase chain reaction, immunoblot, and confocal microscopy analyses were used to study function and expression of ion transporters and cell proliferation. Results Studies with Ussing chambers demonstrated reduced basal and/or adenosine 3′,5′-cyclic monophosphate-mediated electrogenic ion transport in infected colonic tissues, attributable to changes in chloride or sodium transport, depending on the segment studied. The effects of infection were mediated, at least in part, by effector proteins secreted by the bacterial Salmonella pathogenicity island 1- and Salmonella pathogenicity island-2-encoded virulence systems. Infected tissue showed reduced expression of the chloride-bicarbonate exchanger down-regulated in adenoma in surface colonic epithelial cells. Cystic fibrosis transmembrane conductance regulator was internalized in colonic crypt epithelial cells without a change in overall expression levels. Confocal analyses, densitometry, and quantitative polymerase chain reaction revealed that expression of epithelial sodium channel β was reduced in distal colons of Salmonella-infected mice. The changes in transporter expression, localization, and/or function were accompanied by crypt hyperplasia in Salmonella-infected mice. Conclusions Salmonella infection induces diarrhea by altering expression and/or function of transporters that mediate water absorption in the colon, likely reflecting the fact that epithelial cells have less time to differentiate into surface cells when proliferation rates are increased by infection.
AB - Background & Aims Salmonella enterica serovar Typhimurium is an enteropathogen that causes self-limiting diarrhea in healthy individuals, but poses a significant health threat to vulnerable populations. Our understanding of the pathogenesis of Salmonella-induced diarrhea has been hampered by the lack of a suitable mouse model. After a dose of oral kanamycin, Salmonella-infected congenic BALB/c.D2NrampG169 mice, which carry a wild-type Nramp1 gene, develop clear manifestations of diarrhea. We used this model to elucidate the pathophysiology of Salmonella-induced diarrhea. Methods BALB /c.D2 NrampG169 mice were treated with kanamycin and then infected with wild-type or mutant Salmonella by oral gavage. Colon tissues were isolated and Ussing chambers, quantitative polymerase chain reaction, immunoblot, and confocal microscopy analyses were used to study function and expression of ion transporters and cell proliferation. Results Studies with Ussing chambers demonstrated reduced basal and/or adenosine 3′,5′-cyclic monophosphate-mediated electrogenic ion transport in infected colonic tissues, attributable to changes in chloride or sodium transport, depending on the segment studied. The effects of infection were mediated, at least in part, by effector proteins secreted by the bacterial Salmonella pathogenicity island 1- and Salmonella pathogenicity island-2-encoded virulence systems. Infected tissue showed reduced expression of the chloride-bicarbonate exchanger down-regulated in adenoma in surface colonic epithelial cells. Cystic fibrosis transmembrane conductance regulator was internalized in colonic crypt epithelial cells without a change in overall expression levels. Confocal analyses, densitometry, and quantitative polymerase chain reaction revealed that expression of epithelial sodium channel β was reduced in distal colons of Salmonella-infected mice. The changes in transporter expression, localization, and/or function were accompanied by crypt hyperplasia in Salmonella-infected mice. Conclusions Salmonella infection induces diarrhea by altering expression and/or function of transporters that mediate water absorption in the colon, likely reflecting the fact that epithelial cells have less time to differentiate into surface cells when proliferation rates are increased by infection.
KW - Colon
KW - DRA
KW - ENaC
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=84888198757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888198757&partnerID=8YFLogxK
U2 - 10.1053/j.gastro.2013.08.054
DO - 10.1053/j.gastro.2013.08.054
M3 - Article
C2 - 24001788
AN - SCOPUS:84888198757
VL - 145
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 6
ER -