Altered energy metabolism is an additional factor contributing to copper (Cu) deficiency-induced teratogenesis

S. N. Hawk, C. L. Kwik-Uribe, J. Y. Uriu-Hare, G. P. Daston, R. B. Rucker, Carl L Keen

Research output: Contribution to journalArticle

Abstract

Based on our findings of low SOD activity and high oxidative damage, we have suggested that reductions in oxidant defense represent one mechanism contributing to Cu deficiency-induced teratogenesis. In the current work, we tested the hypothesis that an additional mechanism could be impaired enorgy production as a consequence of Cu deficiency associated reductions in cytochrome c oxidase activity. Embryos were removed at gestation day 10 front dams fed either a control (+Cu) or Cu deficient (-Cu) diet (8.0 μg/g and <0.5 μg/g diet, respectively). The +Cu embryos were explanted into -+Cu sera. (19.2 μM) while -Cu embryos wore cultured in -Cu sera (1.2 μM) for 48 h. Kmbryos removed from -Cu dams and cultured in -Cu sera, had significantly lower cytochrome c oxidase activity (50% reduction) than +Cu embryos cultured in +Cu sera. To determine if the lower cytochrome c oxidase activity was functionally significant, ATP concentrations were determined in single embryos using a chemiluminescence assay. AT P concentrations were significantly lower in embryos from -Cu dams that were cultured in -Cu serum than those removed from +Cu dams that were cultured in +Cu serum. These data support the concept that the teratogenicity of Cu deficiency occurs through a number of mechanisms including impairments in energy production and oxidant detente. The role of Cu deficiency-induced alterations in other processes such as angiogenesis and extracellular matrix maturation needs to be investigated.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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    Hawk, S. N., Kwik-Uribe, C. L., Uriu-Hare, J. Y., Daston, G. P., Rucker, R. B., & Keen, C. L. (1997). Altered energy metabolism is an additional factor contributing to copper (Cu) deficiency-induced teratogenesis. FASEB Journal, 11(3).