Altered distribution of basic fibroblast growth factor in diabetic retinopathy

A. Hanneken, E. De Juan, G. A. Lutty, G. M. Fox, S. Schiffer, Leonard M Hjelmeland

Research output: Contribution to journalArticle

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Abstract

Basic fibroblast growth factor (FGF) is a potent endothelial cell mitogen that has been proposed to play a role in proliferative diabetic retinopathy and other neovascular processes. Our understanding of the in vivo role of basic FGF in the pathogenesis of these disorders is limited. We studied the immunolocalization of basic FGF in 16 clinical cases of diabetic retinopathy to determine whether the normal retinal distribution of basic FGF changed during the development of diabetic retinopathy and correlated with the onset of retinal neovascularization. By using monoclonal and affinity-purified polyclonal antibodies against basic FGF and heparan sulfate proteoglycan (HSPG), we found that basic FGF colocalized with HSPG to vascular basement membranes. As the basement membranes thickened during the progression of diabetic retinopathy, the intraretinal stores of immunoreactive basic FGF and HSPG expanded. With the development of neovascularization, the colocalization of basic FGF and HSPG changed; HSPG localized to basement membranes, while basic FGF localized intracellularly, with only minimal basement membrane immunoreactivity. Incubations of the neovascular fronds with exogenous basic FGF demonstrated multiple HSPG glycosaminoglycan-binding sites for basic FGF, indicating that basic FGF had not been released from the matrix of neovascular fronds by heparitanase digestion.

Original languageEnglish (US)
Pages (from-to)1005-1011
Number of pages7
JournalArchives of Ophthalmology
Volume109
Issue number7
StatePublished - 1991

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Fibroblast Growth Factor 2
Diabetic Retinopathy
Heparan Sulfate Proteoglycans
Basement Membrane
Retinal Neovascularization
Normal Distribution
Glycosaminoglycans
Mitogens
Blood Vessels
Digestion
Endothelial Cells
Binding Sites

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Hanneken, A., De Juan, E., Lutty, G. A., Fox, G. M., Schiffer, S., & Hjelmeland, L. M. (1991). Altered distribution of basic fibroblast growth factor in diabetic retinopathy. Archives of Ophthalmology, 109(7), 1005-1011.

Altered distribution of basic fibroblast growth factor in diabetic retinopathy. / Hanneken, A.; De Juan, E.; Lutty, G. A.; Fox, G. M.; Schiffer, S.; Hjelmeland, Leonard M.

In: Archives of Ophthalmology, Vol. 109, No. 7, 1991, p. 1005-1011.

Research output: Contribution to journalArticle

Hanneken, A, De Juan, E, Lutty, GA, Fox, GM, Schiffer, S & Hjelmeland, LM 1991, 'Altered distribution of basic fibroblast growth factor in diabetic retinopathy', Archives of Ophthalmology, vol. 109, no. 7, pp. 1005-1011.
Hanneken A, De Juan E, Lutty GA, Fox GM, Schiffer S, Hjelmeland LM. Altered distribution of basic fibroblast growth factor in diabetic retinopathy. Archives of Ophthalmology. 1991;109(7):1005-1011.
Hanneken, A. ; De Juan, E. ; Lutty, G. A. ; Fox, G. M. ; Schiffer, S. ; Hjelmeland, Leonard M. / Altered distribution of basic fibroblast growth factor in diabetic retinopathy. In: Archives of Ophthalmology. 1991 ; Vol. 109, No. 7. pp. 1005-1011.
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