TY - JOUR
T1 - Alterations of ultrastructural and fission/fusion markers in hepatocyte mitochondria from mice following calorie restriction with different dietary fats
AU - Khraiwesh, Husam
AU - López-Domínguez, José A.
AU - López-Lluch, Guillermo
AU - Navas, Plácido
AU - De Cabo, Rafael
AU - Ramsey, Jon J
AU - Villalba, José M.
AU - González-Reyes, José A.
PY - 2013/9
Y1 - 2013/9
N2 - We analyzed ultrastructural changes and markers of fission/fusion in hepatocyte mitochondria from mice submitted to 40% calorie restriction (CR) for 6 months versus ad-libitum-fed controls. To study the effects of dietary fat under CR, animals were separated into three CR groups with soybean oil (also in controls), fish oil, and lard. CR induced differential changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. The number of cristae per mitochondrion was significantly higher in all CR groups compared with controls. Proteins related to mitochondrial fission (Fis1 and Drp1) increased with CR, but no changes were detected in proteins involved in mitochondrial fusion (Mfn1, Mfn2, and OPA1). Although many of these changes could be attributed to CR regardless of dietary fat, changing membrane lipid composition by different fat sources did modulate the effects of CR on hepatocyte mitochondria.
AB - We analyzed ultrastructural changes and markers of fission/fusion in hepatocyte mitochondria from mice submitted to 40% calorie restriction (CR) for 6 months versus ad-libitum-fed controls. To study the effects of dietary fat under CR, animals were separated into three CR groups with soybean oil (also in controls), fish oil, and lard. CR induced differential changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. The number of cristae per mitochondrion was significantly higher in all CR groups compared with controls. Proteins related to mitochondrial fission (Fis1 and Drp1) increased with CR, but no changes were detected in proteins involved in mitochondrial fusion (Mfn1, Mfn2, and OPA1). Although many of these changes could be attributed to CR regardless of dietary fat, changing membrane lipid composition by different fat sources did modulate the effects of CR on hepatocyte mitochondria.
KW - Calorie restriction
KW - Dietary fat
KW - Hepatocyte
KW - Mice
KW - Mitochondrial fission and fusion
KW - Mitochondrial ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=84882379435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882379435&partnerID=8YFLogxK
U2 - 10.1093/gerona/glt006
DO - 10.1093/gerona/glt006
M3 - Article
C2 - 23403066
AN - SCOPUS:84882379435
VL - 68
SP - 1023
EP - 1034
JO - The journals of gerontology. Series A, Biological sciences and medical sciences
JF - The journals of gerontology. Series A, Biological sciences and medical sciences
SN - 1079-5006
IS - 9
ER -