Alterations of ultrastructural and fission/fusion markers in hepatocyte mitochondria from mice following calorie restriction with different dietary fats

Husam Khraiwesh, José A. López-Domínguez, Guillermo López-Lluch, Plácido Navas, Rafael De Cabo, Jon J Ramsey, José M. Villalba, José A. González-Reyes

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We analyzed ultrastructural changes and markers of fission/fusion in hepatocyte mitochondria from mice submitted to 40% calorie restriction (CR) for 6 months versus ad-libitum-fed controls. To study the effects of dietary fat under CR, animals were separated into three CR groups with soybean oil (also in controls), fish oil, and lard. CR induced differential changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. The number of cristae per mitochondrion was significantly higher in all CR groups compared with controls. Proteins related to mitochondrial fission (Fis1 and Drp1) increased with CR, but no changes were detected in proteins involved in mitochondrial fusion (Mfn1, Mfn2, and OPA1). Although many of these changes could be attributed to CR regardless of dietary fat, changing membrane lipid composition by different fat sources did modulate the effects of CR on hepatocyte mitochondria.

Original languageEnglish (US)
Pages (from-to)1023-1034
Number of pages12
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume68
Issue number9
DOIs
StatePublished - Sep 2013

Keywords

  • Calorie restriction
  • Dietary fat
  • Hepatocyte
  • Mice
  • Mitochondrial fission and fusion
  • Mitochondrial ultrastructure

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology
  • Medicine(all)

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