Alterations in the jejunal mucosal permeability during varying periods of ischemia and reperfusion in the horse

N. J. Vatistas, J. R. Snyder, M. J. Woliner, Jorge Nieto, D. N. Granger, S. Alexander

Research output: Contribution to journalArticle

Abstract

Certain conditions causing acute abdominal crises in horses are characterized by jejunal ischemia and subsequent reperfusion. The aim of this study was to determine the changes in mucosal permeability of the jejunum of horses during ischemia and reperfusion. Following general anesthesia, the artery and vein supplying an approximately 25cm length of jejunum were catheterized. The segment was perfused with whole blood using an isolated circuit. Blood was oxygenated employing an ECMO®. The lumen was perfused with lactated ringers solution, and the lumenal contents collected. The tissue, blood and perfusate were maintained at 37°C, and blood gases, pH and electrolytes were monitored and maintained at physiologic values. Baseline values were obtained during an initial period of normal blood flow. Blood flow was reduced to 20% of baseline for 20, 40, 60 or 90 minutes, and subsequently reperfused for 60 minutes. Serum and lumenal albumin concentration were determined using ELISA. Mucosal permeability was determined from the amount of albumin in the lumenal contents. Histologically, increasing length of ischemia produced greater degree of mucosal damage. Mucosal permeability increased during ischemia and continued to increased during reperfusion. The magnitude of the increase was proportional to the degree of ischemia. This model may be useful in determining the alterations in mucosal permeability in experimental and clinical material from horses and other species.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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    Vatistas, N. J., Snyder, J. R., Woliner, M. J., Nieto, J., Granger, D. N., & Alexander, S. (1997). Alterations in the jejunal mucosal permeability during varying periods of ischemia and reperfusion in the horse. FASEB Journal, 11(3).