Abstract
Chromatin remodeling plays a key role in the transcriptional activation of regulatory factors in the liver in response to a variety of stress signals. The effects of burn injury on histone expression and its modification were investigated in this study. Liver tissues collected after a flame burn injury were subjected to RT-PCR and Western blot analyses of histone regulation. There was a marked induction of histone H3-D variant mRNA at 3 and 6 h. In contrast, histone H2A.2 variant mRNA had a downregulation at 3 days. No apparent changes were noted in other histone variants examined. Western blot analysis revealed a downregulation of all 5 histone subtypes (H1, H2A, H2B, H3, and H4) at 1 day and there was a subsequent induction of H1 and H2A subtypes at 3 days after injury. There was an induction of modified forms (phospho-, acetyl-, and dimethyl-) of histone H3 subtype at day 3. Furthermore, a transient elevation in PCNA (proliferating cell nuclear antigen) levels was apparent in the liver at day 3, which parallels the induction of phospho-histone H3, which is a mitosis marker. These findings suggest that histones participate in a cascade of events associated with phenotypic alterations in the liver after injury.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 256-264 |
| Number of pages | 9 |
| Journal | Experimental and Molecular Pathology |
| Volume | 75 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2003 |
Fingerprint
Keywords
- Burn injury
- Histone
- Liver
- Modification
- Proliferating cell nuclear antigen
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Pathology and Forensic Medicine
Cite this
Alterations in the expression and modification of histones in the liver after injury. / Jeong, Jayoung; Adamson, Lee K.; Hatam, Robin; Greenhalgh, David G; Cho, Kiho.
In: Experimental and Molecular Pathology, Vol. 75, No. 3, 12.2003, p. 256-264.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alterations in the expression and modification of histones in the liver after injury
AU - Jeong, Jayoung
AU - Adamson, Lee K.
AU - Hatam, Robin
AU - Greenhalgh, David G
AU - Cho, Kiho
PY - 2003/12
Y1 - 2003/12
N2 - Chromatin remodeling plays a key role in the transcriptional activation of regulatory factors in the liver in response to a variety of stress signals. The effects of burn injury on histone expression and its modification were investigated in this study. Liver tissues collected after a flame burn injury were subjected to RT-PCR and Western blot analyses of histone regulation. There was a marked induction of histone H3-D variant mRNA at 3 and 6 h. In contrast, histone H2A.2 variant mRNA had a downregulation at 3 days. No apparent changes were noted in other histone variants examined. Western blot analysis revealed a downregulation of all 5 histone subtypes (H1, H2A, H2B, H3, and H4) at 1 day and there was a subsequent induction of H1 and H2A subtypes at 3 days after injury. There was an induction of modified forms (phospho-, acetyl-, and dimethyl-) of histone H3 subtype at day 3. Furthermore, a transient elevation in PCNA (proliferating cell nuclear antigen) levels was apparent in the liver at day 3, which parallels the induction of phospho-histone H3, which is a mitosis marker. These findings suggest that histones participate in a cascade of events associated with phenotypic alterations in the liver after injury.
AB - Chromatin remodeling plays a key role in the transcriptional activation of regulatory factors in the liver in response to a variety of stress signals. The effects of burn injury on histone expression and its modification were investigated in this study. Liver tissues collected after a flame burn injury were subjected to RT-PCR and Western blot analyses of histone regulation. There was a marked induction of histone H3-D variant mRNA at 3 and 6 h. In contrast, histone H2A.2 variant mRNA had a downregulation at 3 days. No apparent changes were noted in other histone variants examined. Western blot analysis revealed a downregulation of all 5 histone subtypes (H1, H2A, H2B, H3, and H4) at 1 day and there was a subsequent induction of H1 and H2A subtypes at 3 days after injury. There was an induction of modified forms (phospho-, acetyl-, and dimethyl-) of histone H3 subtype at day 3. Furthermore, a transient elevation in PCNA (proliferating cell nuclear antigen) levels was apparent in the liver at day 3, which parallels the induction of phospho-histone H3, which is a mitosis marker. These findings suggest that histones participate in a cascade of events associated with phenotypic alterations in the liver after injury.
KW - Burn injury
KW - Histone
KW - Liver
KW - Modification
KW - Proliferating cell nuclear antigen
UR - http://www.scopus.com/inward/record.url?scp=0242594496&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242594496&partnerID=8YFLogxK
U2 - 10.1016/S0014-4800(03)00095-9
DO - 10.1016/S0014-4800(03)00095-9
M3 - Article
VL - 75
SP - 256
EP - 264
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
SN - 0014-4800
IS - 3
ER -