Alterations in the expression and modification of histones in the liver after injury

Jayoung Jeong, Lee K. Adamson, Robin Hatam, David G Greenhalgh, Kiho Cho

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Chromatin remodeling plays a key role in the transcriptional activation of regulatory factors in the liver in response to a variety of stress signals. The effects of burn injury on histone expression and its modification were investigated in this study. Liver tissues collected after a flame burn injury were subjected to RT-PCR and Western blot analyses of histone regulation. There was a marked induction of histone H3-D variant mRNA at 3 and 6 h. In contrast, histone H2A.2 variant mRNA had a downregulation at 3 days. No apparent changes were noted in other histone variants examined. Western blot analysis revealed a downregulation of all 5 histone subtypes (H1, H2A, H2B, H3, and H4) at 1 day and there was a subsequent induction of H1 and H2A subtypes at 3 days after injury. There was an induction of modified forms (phospho-, acetyl-, and dimethyl-) of histone H3 subtype at day 3. Furthermore, a transient elevation in PCNA (proliferating cell nuclear antigen) levels was apparent in the liver at day 3, which parallels the induction of phospho-histone H3, which is a mitosis marker. These findings suggest that histones participate in a cascade of events associated with phenotypic alterations in the liver after injury.

Original languageEnglish (US)
Pages (from-to)256-264
Number of pages9
JournalExperimental and Molecular Pathology
Volume75
Issue number3
DOIs
StatePublished - Dec 2003

Keywords

  • Burn injury
  • Histone
  • Liver
  • Modification
  • Proliferating cell nuclear antigen

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

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