Alterations in maternal-fetal cellular trafficking after fetal surgery

Payam Saadai, Tzong Hae Lee, Geoanna Bautista, Kelly D. Gonzales, Amar Nijagal, Michael P. Busch, Chong Jai Kim, Roberto Romero, Hanmin Lee, Shinjiro Hirose, Larry Rand, Douglas Miniati, Diana L Farmer, Tippi C. MacKenzie

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background/Purpose: Bidirectional trafficking of cells between the mother and the fetus is routine in pregnancy and a component of maternal-fetal tolerance. Changes in fetal-to-maternal cellular trafficking have been reported in prenatal complications, but maternal-to-fetal trafficking has never been studied in the context of fetal intervention. We hypothesized that patients undergoing open fetal surgery would have altered maternal-fetal cellular trafficking. Methods: Cellular trafficking was analyzed in patients with myelomeningocele (MMC) who underwent open fetal surgical repair (n = 5), patients with MMC who had routine postnatal repair (n = 6), and healthy control healthy patients (n = 9). As an additional control for the fetal operation, trafficking was also analyzed in patients who were delivered by an ex utero intrapartum treatment procedure (n = 6). Microchimerism in maternal and cord blood was determined using quantitative real-time polymerase chain reaction for nonshared alleles. Results: Maternal-to-fetal trafficking was significantly increased in patients who underwent open fetal surgery for MMC compared with healthy controls, patients who underwent postnatal MMC repair, and patients who underwent ex utero intrapartum treatment. There were no differences in fetal-to-maternal cell trafficking among groups. Conclusion: Patients undergoing open fetal surgery for MMC have elevated levels of maternal microchimerism. These results suggest altered trafficking and/or increased proliferation of maternal cells in fetal blood and may have important implications for preterm labor.

Original languageEnglish (US)
Pages (from-to)1089-1094
Number of pages6
JournalJournal of pediatric surgery
Volume47
Issue number6
DOIs
StatePublished - Jun 1 2012
Externally publishedYes

Fingerprint

Mothers
Meningomyelocele
Chimerism
Fetal Blood
Premature Obstetric Labor
Real-Time Polymerase Chain Reaction
Fetus
Stem Cells
Alleles
Cell Proliferation
Pregnancy
Therapeutics

Keywords

  • EXIT
  • Fetal surgery
  • Maternal-fetal cellular trafficking
  • Microchimerism
  • Myelomeningocele
  • Preterm labor
  • Spina bifida

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Saadai, P., Lee, T. H., Bautista, G., Gonzales, K. D., Nijagal, A., Busch, M. P., ... MacKenzie, T. C. (2012). Alterations in maternal-fetal cellular trafficking after fetal surgery. Journal of pediatric surgery, 47(6), 1089-1094. https://doi.org/10.1016/j.jpedsurg.2012.03.012

Alterations in maternal-fetal cellular trafficking after fetal surgery. / Saadai, Payam; Lee, Tzong Hae; Bautista, Geoanna; Gonzales, Kelly D.; Nijagal, Amar; Busch, Michael P.; Kim, Chong Jai; Romero, Roberto; Lee, Hanmin; Hirose, Shinjiro; Rand, Larry; Miniati, Douglas; Farmer, Diana L; MacKenzie, Tippi C.

In: Journal of pediatric surgery, Vol. 47, No. 6, 01.06.2012, p. 1089-1094.

Research output: Contribution to journalArticle

Saadai, P, Lee, TH, Bautista, G, Gonzales, KD, Nijagal, A, Busch, MP, Kim, CJ, Romero, R, Lee, H, Hirose, S, Rand, L, Miniati, D, Farmer, DL & MacKenzie, TC 2012, 'Alterations in maternal-fetal cellular trafficking after fetal surgery', Journal of pediatric surgery, vol. 47, no. 6, pp. 1089-1094. https://doi.org/10.1016/j.jpedsurg.2012.03.012
Saadai, Payam ; Lee, Tzong Hae ; Bautista, Geoanna ; Gonzales, Kelly D. ; Nijagal, Amar ; Busch, Michael P. ; Kim, Chong Jai ; Romero, Roberto ; Lee, Hanmin ; Hirose, Shinjiro ; Rand, Larry ; Miniati, Douglas ; Farmer, Diana L ; MacKenzie, Tippi C. / Alterations in maternal-fetal cellular trafficking after fetal surgery. In: Journal of pediatric surgery. 2012 ; Vol. 47, No. 6. pp. 1089-1094.
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abstract = "Background/Purpose: Bidirectional trafficking of cells between the mother and the fetus is routine in pregnancy and a component of maternal-fetal tolerance. Changes in fetal-to-maternal cellular trafficking have been reported in prenatal complications, but maternal-to-fetal trafficking has never been studied in the context of fetal intervention. We hypothesized that patients undergoing open fetal surgery would have altered maternal-fetal cellular trafficking. Methods: Cellular trafficking was analyzed in patients with myelomeningocele (MMC) who underwent open fetal surgical repair (n = 5), patients with MMC who had routine postnatal repair (n = 6), and healthy control healthy patients (n = 9). As an additional control for the fetal operation, trafficking was also analyzed in patients who were delivered by an ex utero intrapartum treatment procedure (n = 6). Microchimerism in maternal and cord blood was determined using quantitative real-time polymerase chain reaction for nonshared alleles. Results: Maternal-to-fetal trafficking was significantly increased in patients who underwent open fetal surgery for MMC compared with healthy controls, patients who underwent postnatal MMC repair, and patients who underwent ex utero intrapartum treatment. There were no differences in fetal-to-maternal cell trafficking among groups. Conclusion: Patients undergoing open fetal surgery for MMC have elevated levels of maternal microchimerism. These results suggest altered trafficking and/or increased proliferation of maternal cells in fetal blood and may have important implications for preterm labor.",
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AU - Saadai, Payam

AU - Lee, Tzong Hae

AU - Bautista, Geoanna

AU - Gonzales, Kelly D.

AU - Nijagal, Amar

AU - Busch, Michael P.

AU - Kim, Chong Jai

AU - Romero, Roberto

AU - Lee, Hanmin

AU - Hirose, Shinjiro

AU - Rand, Larry

AU - Miniati, Douglas

AU - Farmer, Diana L

AU - MacKenzie, Tippi C.

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N2 - Background/Purpose: Bidirectional trafficking of cells between the mother and the fetus is routine in pregnancy and a component of maternal-fetal tolerance. Changes in fetal-to-maternal cellular trafficking have been reported in prenatal complications, but maternal-to-fetal trafficking has never been studied in the context of fetal intervention. We hypothesized that patients undergoing open fetal surgery would have altered maternal-fetal cellular trafficking. Methods: Cellular trafficking was analyzed in patients with myelomeningocele (MMC) who underwent open fetal surgical repair (n = 5), patients with MMC who had routine postnatal repair (n = 6), and healthy control healthy patients (n = 9). As an additional control for the fetal operation, trafficking was also analyzed in patients who were delivered by an ex utero intrapartum treatment procedure (n = 6). Microchimerism in maternal and cord blood was determined using quantitative real-time polymerase chain reaction for nonshared alleles. Results: Maternal-to-fetal trafficking was significantly increased in patients who underwent open fetal surgery for MMC compared with healthy controls, patients who underwent postnatal MMC repair, and patients who underwent ex utero intrapartum treatment. There were no differences in fetal-to-maternal cell trafficking among groups. Conclusion: Patients undergoing open fetal surgery for MMC have elevated levels of maternal microchimerism. These results suggest altered trafficking and/or increased proliferation of maternal cells in fetal blood and may have important implications for preterm labor.

AB - Background/Purpose: Bidirectional trafficking of cells between the mother and the fetus is routine in pregnancy and a component of maternal-fetal tolerance. Changes in fetal-to-maternal cellular trafficking have been reported in prenatal complications, but maternal-to-fetal trafficking has never been studied in the context of fetal intervention. We hypothesized that patients undergoing open fetal surgery would have altered maternal-fetal cellular trafficking. Methods: Cellular trafficking was analyzed in patients with myelomeningocele (MMC) who underwent open fetal surgical repair (n = 5), patients with MMC who had routine postnatal repair (n = 6), and healthy control healthy patients (n = 9). As an additional control for the fetal operation, trafficking was also analyzed in patients who were delivered by an ex utero intrapartum treatment procedure (n = 6). Microchimerism in maternal and cord blood was determined using quantitative real-time polymerase chain reaction for nonshared alleles. Results: Maternal-to-fetal trafficking was significantly increased in patients who underwent open fetal surgery for MMC compared with healthy controls, patients who underwent postnatal MMC repair, and patients who underwent ex utero intrapartum treatment. There were no differences in fetal-to-maternal cell trafficking among groups. Conclusion: Patients undergoing open fetal surgery for MMC have elevated levels of maternal microchimerism. These results suggest altered trafficking and/or increased proliferation of maternal cells in fetal blood and may have important implications for preterm labor.

KW - EXIT

KW - Fetal surgery

KW - Maternal-fetal cellular trafficking

KW - Microchimerism

KW - Myelomeningocele

KW - Preterm labor

KW - Spina bifida

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JO - Journal of Pediatric Surgery

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