Alterations in expression and structure of the DNA repair gene XRCC1

Heahyun Yoo, Li Li, Peter G. Sacks, Larry H. Thompson, Frederick F. Becker, John Y H Chan

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The repair-associated gene XRCC1 was previously cloned by complementing the hamster mutant EM9, which has a high rate of spontaneous SCE and hypersensitivity to DNA damaging agents. In analyzing XRCC1 gene expression, similar levels of steady-state mRNA were found in normal cells, Bloom's syndrome cells with altered SCE, and in squamous carcinoma cells with differential X-ray sensitivity. An EcoRI restriction fragment-length polymorphism previously identified in XRCC1 did not correlate with the repair phenotypes of these cells. The mRNA of XRCC1 decreased to 20-40% after treatment of cells with a DNA damaging agent. XRCC1 also showed tissue specific expression in rats. The mRNA levels were high in testis (7-8 fold), ovary (3-4 fold) and brain (4-5 fold), when compared with those in intestine, liver and spleen (1-2 fold). These data and the high levels of XRCC1 protein detected in testis indicate that XRCC1 may play an important role in DNA processing during meiogenesis and recombination in germ cells.

Original languageEnglish (US)
Pages (from-to)900-910
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
StatePublished - Jul 31 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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