Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy

G. P. Hasenfuss, L. A. Mulieri, B. J. Leavitt, P. D. Allen, J. R. Haeberle, N. R. Alpert

Research output: Contribution to journalArticle

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Abstract

Myocardial failure in dilated cardiomyopathy may result from subcellular alterations in contractile protein function, excitation-contraction coupling processes, or recovery metabolism. We used isometric force and heat measurements to quantitatively investigate these subcellular systems in intact left ventricular muscle strips from nonfailing human hearts (n=14) and from hearts with end-stage failing dilated cardiomyopathy (n=13). In the failing myocardium, peak isometric twitch tension, maximum rate of tension rise, and maximum rate of relaxation were reduced by 46% (p=0.013), 51% (p=0.003), and 46% (p=0.018), respectively (37°C, 60 beats per minute). Tension-dependent heat, reflecting the number of crossbridge interactions during the isometric twitch, was reduced by 61% in the failing myocardium (p=0.006). In terms of the individual crossbridge cycle, the average crossbridge force-time integral was increased by 33% (p=0.04) in the failing myocardium. In the nonfailing myocardium, the crossbridge force-time integral was positively correlated with the patient's age (r=0.86, p

Original languageEnglish (US)
Pages (from-to)1225-1232
Number of pages8
JournalCirculation Research
Volume70
Issue number6
StatePublished - 1992
Externally publishedYes

Fingerprint

Excitation Contraction Coupling
Dilated Cardiomyopathy
Myocardium
Hot Temperature
Contractile Proteins
Heart Failure
Muscles

Keywords

  • calcium cycling
  • crossbridges
  • dilated cardiomyopathy myocardial failure
  • human myocardium

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Hasenfuss, G. P., Mulieri, L. A., Leavitt, B. J., Allen, P. D., Haeberle, J. R., & Alpert, N. R. (1992). Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy. Circulation Research, 70(6), 1225-1232.

Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy. / Hasenfuss, G. P.; Mulieri, L. A.; Leavitt, B. J.; Allen, P. D.; Haeberle, J. R.; Alpert, N. R.

In: Circulation Research, Vol. 70, No. 6, 1992, p. 1225-1232.

Research output: Contribution to journalArticle

Hasenfuss, GP, Mulieri, LA, Leavitt, BJ, Allen, PD, Haeberle, JR & Alpert, NR 1992, 'Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy', Circulation Research, vol. 70, no. 6, pp. 1225-1232.
Hasenfuss GP, Mulieri LA, Leavitt BJ, Allen PD, Haeberle JR, Alpert NR. Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy. Circulation Research. 1992;70(6):1225-1232.
Hasenfuss, G. P. ; Mulieri, L. A. ; Leavitt, B. J. ; Allen, P. D. ; Haeberle, J. R. ; Alpert, N. R. / Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy. In: Circulation Research. 1992 ; Vol. 70, No. 6. pp. 1225-1232.
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