Alteration of chloride secretion across canine tracheal epithelium by lipoxygenase products of arachidonic acid

G. D. Leikauf, I. F. Ueki, Jonathan Widdicombe, J. A. Nadel

Research output: Chapter in Book/Report/Conference proceedingChapter

48 Scopus citations

Abstract

We examined whether the leukotrienes (LT) B4, C4, D4, and E4, and 5-, 12-, and 15-hydroxyeicosatetraenoic (HETE) acids alter the electrical properties of canine tracheal epithelium. Short-circuit current (I(sc)) increased by 20.3 ± 4.3 and 25.9 ± 6.5 μA/cm2 after LTC4 and LTD4 addition, was unchanged following LTB4, LTE4, 5-, or 15-HETE addition, and was decreased by 10 ± 3% after 12-HETE addition. LTC4- and LTD4-induced increases in I(sc) were dependent on the presence of Cl and were equal to the increases in net 36Cl flux toward the mucosa. The cyclooxygenase inhibitor indomethacin (10-5 M) or the phospholipase inhibitor mepacrine (10-5 M) diminished increases in I(sc). The release rate of prostaglandins E2 and F(2α) and thromboxane B2 rose from 8.2 ± 2.3, 2.3 ± 0.5, and 1.1 ± 0.5 to 15.5 ± 3.3, 6.0 ± 1.4, and 4.0 ± 0.8 ng.cm-2.h-1, respectively, after LTC4 addition, and from 6.9 ± 1.4, 2.5 ± 1.1, and 1.7 ± 1.3 to 20.2 ± 3.1, 10.5 ± 3.1, and 5.2 ± 2.4 ng.cm-2.h-1, respectively, after LTD4 addition. The release rates of 6-keto-prostaglandin F(1α) were unchanged. A thromboxane A2 mimetic, U46619, failed to increase I(sc) and thromboxane synthetase inhibitors OKY-046 and dazmegrel failed to inhibit the induced increases in I(sc), suggesting that thromboxane has little effect on I(sc). Thus lipoxygenase products of arachidonic acid can modulate Cl secretion by the canine tracheal epithelium and do so through the release of cyclooxygenase products, including prostaglandins E2 and F(2α).

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume250
Edition1
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Leikauf, G. D., Ueki, I. F., Widdicombe, J., & Nadel, J. A. (1986). Alteration of chloride secretion across canine tracheal epithelium by lipoxygenase products of arachidonic acid. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (1 ed., Vol. 250)