Abstract
Hepatoma cells derived from The Jackson Laboratory mouse hepatoma BW7756 synthesized alpha fetoprotein (AFP) in vitro. The AFP was immunologically identical to that circulating in the sera of hepatoma bearing mice. An in vitro cytotoxic effect of rabbit anti serum to AFP was studied in hepatoma cells obtained both from fresh cell suspensions and short term cell culture. The use of intact and/or inactivated anti AFP serum inhibited the growth of the AFP producing cells. The cytotoxic effects of the antiserum depended on exposure time and serum concentration. The cytotoxicity was complement independent, as demonstrated by studies with heat deactivated serum devoid of extrinsic complement. The control target cells included fresh cell suspensions of normal mouse liver and mouse muscle fibroblasts grown in short term culture. Specificity of the antisera for the target cells was demonstrated by absorption with purified mouse AFP. The results could be explained by the presence of AFP on the hepatoma cell surface.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1361-1367 |
| Number of pages | 7 |
| Journal | Journal of the National Cancer Institute |
| Volume | 54 |
| Issue number | 6 |
| State | Published - 1975 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Alpha fetoprotein : effect of heterologous antiserum on hepatoma cells in vitro. / Mizejewski, G. J.; Young, S. R.; Allen, Roblee P.
In: Journal of the National Cancer Institute, Vol. 54, No. 6, 1975, p. 1361-1367.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alpha fetoprotein
T2 - effect of heterologous antiserum on hepatoma cells in vitro
AU - Mizejewski, G. J.
AU - Young, S. R.
AU - Allen, Roblee P
PY - 1975
Y1 - 1975
N2 - Hepatoma cells derived from The Jackson Laboratory mouse hepatoma BW7756 synthesized alpha fetoprotein (AFP) in vitro. The AFP was immunologically identical to that circulating in the sera of hepatoma bearing mice. An in vitro cytotoxic effect of rabbit anti serum to AFP was studied in hepatoma cells obtained both from fresh cell suspensions and short term cell culture. The use of intact and/or inactivated anti AFP serum inhibited the growth of the AFP producing cells. The cytotoxic effects of the antiserum depended on exposure time and serum concentration. The cytotoxicity was complement independent, as demonstrated by studies with heat deactivated serum devoid of extrinsic complement. The control target cells included fresh cell suspensions of normal mouse liver and mouse muscle fibroblasts grown in short term culture. Specificity of the antisera for the target cells was demonstrated by absorption with purified mouse AFP. The results could be explained by the presence of AFP on the hepatoma cell surface.
AB - Hepatoma cells derived from The Jackson Laboratory mouse hepatoma BW7756 synthesized alpha fetoprotein (AFP) in vitro. The AFP was immunologically identical to that circulating in the sera of hepatoma bearing mice. An in vitro cytotoxic effect of rabbit anti serum to AFP was studied in hepatoma cells obtained both from fresh cell suspensions and short term cell culture. The use of intact and/or inactivated anti AFP serum inhibited the growth of the AFP producing cells. The cytotoxic effects of the antiserum depended on exposure time and serum concentration. The cytotoxicity was complement independent, as demonstrated by studies with heat deactivated serum devoid of extrinsic complement. The control target cells included fresh cell suspensions of normal mouse liver and mouse muscle fibroblasts grown in short term culture. Specificity of the antisera for the target cells was demonstrated by absorption with purified mouse AFP. The results could be explained by the presence of AFP on the hepatoma cell surface.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016785817&partnerID=8YFLogxK
M3 - Article
C2 - 48552
AN - SCOPUS:0016785817
VL - 54
SP - 1361
EP - 1367
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 6
ER -