Alpha fetoprotein: effect of heterologous antiserum on hepatoma cells in vitro

G. J. Mizejewski, S. R. Young, Roblee P Allen

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Hepatoma cells derived from The Jackson Laboratory mouse hepatoma BW7756 synthesized alpha fetoprotein (AFP) in vitro. The AFP was immunologically identical to that circulating in the sera of hepatoma bearing mice. An in vitro cytotoxic effect of rabbit anti serum to AFP was studied in hepatoma cells obtained both from fresh cell suspensions and short term cell culture. The use of intact and/or inactivated anti AFP serum inhibited the growth of the AFP producing cells. The cytotoxic effects of the antiserum depended on exposure time and serum concentration. The cytotoxicity was complement independent, as demonstrated by studies with heat deactivated serum devoid of extrinsic complement. The control target cells included fresh cell suspensions of normal mouse liver and mouse muscle fibroblasts grown in short term culture. Specificity of the antisera for the target cells was demonstrated by absorption with purified mouse AFP. The results could be explained by the presence of AFP on the hepatoma cell surface.

Original languageEnglish (US)
Pages (from-to)1361-1367
Number of pages7
JournalJournal of the National Cancer Institute
Volume54
Issue number6
StatePublished - 1975
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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