Alpha and beta adrenergic receptors modulate keratinocyte migration

Hsin Ya Yang, Pieter Steenhuis, Aaron M. Glucksman, Zhanna Gurenko, Thi Dinh La, R. Rivkah Isseroff

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Keratinocyte migration into skin wounds is the step of the healing process that correlates with the wound closure rate. Keratinocyte migration, and wound epithelialization are decreased when beta 2-adrenergic receptors (B2AR) are activated by 1 μM epinephrine/ adrenaline, resulting in delayed wound healing in human and mouse skin. In the present study, we found paradoxically, that in a subset of keratinocyte strains exposure to low concentrations of epinephrine (0.1 nM) increased, rather than decreased, their migratory rate. We find that both the alpha- and the beta-adrenergic receptors are expressed in human keratinocytes, and expression of alpha-2 AR subtypes demonstrated for the first time. Therefore, we tested if the alpha-AR could be modulating the increased migratory response observed in these cell strains. By using specific inhibitors to alpha-AR, we demonstrated that blocking A2B-AR could reverse the rapid cell migration induced by the 0.1 nM epinephrine. Phosphorylation of ERK was elevated after 1–10 minutes of the low epinephrine treatment and the A2B-AR inhibitor blocked the ERK phosphorylation. The results suggest that both the A2B-AR and B2AR mediate keratinocyte migration, in which with a low level of epinephrine treatment, A2B-AR could alter the B2AR signals and regulate the migration rate.

Original languageEnglish (US)
Article numbere0253139
JournalPloS one
Volume16
Issue number7 July
DOIs
StatePublished - Jul 2021
Externally publishedYes

ASJC Scopus subject areas

  • General

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