Abstract
Scope: The aim of this study is to investigate the signaling pathways by which allyl isothiocyanate (AITC) reduces adipocyte differentiation and the efficacy of AITC in suppressing galectin-12 levels as a therapeutic for high fat diet (HFD)-induced obesity. Methods and results: AITC presents anti-adipogenic effects on 3T3-L1 cells by decreasing lipid droplet accumulation in a dose-dependent manner. AITC suppresses 3T3-L1 differentiation into adipocytes by decreasing galectin-12 expression and by downregulating key adipogenic transcription factors. AITC influences the expression of 3T3-L1 pre-adipocytes by modulating adipokine expression (leptin and resistin) and by regulating the protein kinase B (PKB/Akt)/cAMP response element-binding protein (CREB) pathway. In HFD-fed mice, oral administration of AITC reduces the body weight, accumulation of lipid droplets in the liver, and white adipocyte size. Conclusion: In summary, the results indicate that AITC inhibits adipocyte differentiation by suppressing galectin-12 levels in 3T3L1 cells and has antiobesity effects in HFD-fed mice.
| Original language | English (US) |
|---|---|
| Article number | 1700616 |
| Journal | Molecular Nutrition and Food Research |
| Volume | 62 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 1 2018 |
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Keywords
- 3T3-L1
- adipocytes
- Allyl isothiocyanate
- galectin-12
- obesity
ASJC Scopus subject areas
- Biotechnology
- Food Science
Cite this
Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12. / Lo, Chia Wen; Chen, Chih Sheng; Chen, Ying Chi; Hsu, Yu An; Huang, Chi Chun; Chang, Ching Yao; Lin, Chao Jen; Lin, Cheng Wen; Lin, Hui Ju; Liu, Fu-Tong; Wan, Lei.
In: Molecular Nutrition and Food Research, Vol. 62, No. 6, 1700616, 01.03.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12
AU - Lo, Chia Wen
AU - Chen, Chih Sheng
AU - Chen, Ying Chi
AU - Hsu, Yu An
AU - Huang, Chi Chun
AU - Chang, Ching Yao
AU - Lin, Chao Jen
AU - Lin, Cheng Wen
AU - Lin, Hui Ju
AU - Liu, Fu-Tong
AU - Wan, Lei
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Scope: The aim of this study is to investigate the signaling pathways by which allyl isothiocyanate (AITC) reduces adipocyte differentiation and the efficacy of AITC in suppressing galectin-12 levels as a therapeutic for high fat diet (HFD)-induced obesity. Methods and results: AITC presents anti-adipogenic effects on 3T3-L1 cells by decreasing lipid droplet accumulation in a dose-dependent manner. AITC suppresses 3T3-L1 differentiation into adipocytes by decreasing galectin-12 expression and by downregulating key adipogenic transcription factors. AITC influences the expression of 3T3-L1 pre-adipocytes by modulating adipokine expression (leptin and resistin) and by regulating the protein kinase B (PKB/Akt)/cAMP response element-binding protein (CREB) pathway. In HFD-fed mice, oral administration of AITC reduces the body weight, accumulation of lipid droplets in the liver, and white adipocyte size. Conclusion: In summary, the results indicate that AITC inhibits adipocyte differentiation by suppressing galectin-12 levels in 3T3L1 cells and has antiobesity effects in HFD-fed mice.
AB - Scope: The aim of this study is to investigate the signaling pathways by which allyl isothiocyanate (AITC) reduces adipocyte differentiation and the efficacy of AITC in suppressing galectin-12 levels as a therapeutic for high fat diet (HFD)-induced obesity. Methods and results: AITC presents anti-adipogenic effects on 3T3-L1 cells by decreasing lipid droplet accumulation in a dose-dependent manner. AITC suppresses 3T3-L1 differentiation into adipocytes by decreasing galectin-12 expression and by downregulating key adipogenic transcription factors. AITC influences the expression of 3T3-L1 pre-adipocytes by modulating adipokine expression (leptin and resistin) and by regulating the protein kinase B (PKB/Akt)/cAMP response element-binding protein (CREB) pathway. In HFD-fed mice, oral administration of AITC reduces the body weight, accumulation of lipid droplets in the liver, and white adipocyte size. Conclusion: In summary, the results indicate that AITC inhibits adipocyte differentiation by suppressing galectin-12 levels in 3T3L1 cells and has antiobesity effects in HFD-fed mice.
KW - 3T3-L1
KW - adipocytes
KW - Allyl isothiocyanate
KW - galectin-12
KW - obesity
UR - http://www.scopus.com/inward/record.url?scp=85043454670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85043454670&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201700616
DO - 10.1002/mnfr.201700616
M3 - Article
VL - 62
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
IS - 6
M1 - 1700616
ER -