Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen

Matthew D. Collins, Robert Higgins, Serge Messier, Madeleine Fortin, Roger A. Hutson, Paul A. Lawson, Enevold Falsen

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

An unknown Gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a directly cross-linked cell wall murein based on L-lysine and a DNA G+C content of 39 mol%. Comparative 16S rRNA gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, albeit loosely, to Alkalibacterium olivapovliticus, Alloiococcus otitis, Dolosigranulum pigrum and related organisms, in the low-G+C-content Gram-positive bacteria. However, sequence divergence values of > 11 % from these recognized taxa clearly indicated that the novel bacterium represents a separate genus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium from pig semen be classified as a new genus and species, Allofustis seminis gen. nov., sp. nov. The type strain is strain 01-570-1T (=CCUG 45438T=CIP 107425T).

Original languageEnglish (US)
Pages (from-to)811-814
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume53
Issue number3
DOIs
StatePublished - May 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

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