Allergic asthmatics show divergent lipid mediator profiles from healthy controls both at baseline and following birch pollen provocation

Susanna L. Lundström, Jun Yang, Henrik J. Källberg, Sarah Thunberg, Guro Gafvelin, Jesper Z. Haeggström, Reidar Grönneberg, Johan Grunewald, Marianne van Hage, Bruce D. Hammock, Anders Eklund, Åsa M. Wheelock, Craig E. Wheelock

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: Asthma is a respiratory tract disorder characterized by airway hyper-reactivity and chronic inflammation. Allergic asthma is associated with the production of allergen-specific IgE and expansion of allergen-specific T-cell populations. Progression of allergic inflammation is driven by T-helper type 2 (Th2) mediators and is associated with alterations in the levels of lipid mediators. Objectives: Responses of the respiratory system to birch allergen provocation in allergic asthmatics were investigated. Eicosanoids and other oxylipins were quantified in the bronchoalveolar lumen to provide a measure of shifts in lipid mediators associated with allergen challenge in allergic asthmatics. Methods: Eighty-seven lipid mediators representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS following off-line extraction of bronchoalveolar lavage fluid (BALF). Multivariate statistics using OPLS were employed to interrogate acquired oxylipin data in combination with immunological markers. Results: Thirty-two oxylipins were quantified, with baseline asthmatics possessing a different oxylipin profile relative to healthy individuals that became more distinct following allergen provocation. The most prominent differences included 15-LOX-derived ω-3 and ω-6 oxylipins. Shared-and-Unique-Structures (SUS)-plot modeling showed a correlation (R 2 = 0.7) between OPLS models for baseline asthmatics (R 2Y[cum] = 0.87, Q 2[cum] = 0.51) and allergen-provoked asthmatics (R 2Y[cum] = 0.95, Q 2[cum] = 0.73), with the majority of quantified lipid mediators and cytokines contributing equally to both groups. Unique structures for allergen provocation included leukotrienes (LTB 4 and 6-trans-LTB 4), CYP-derivatives of linoleic acid (epoxides/diols), and IL-10. Conclusions: Differences in asthmatic relative to healthy profiles suggest a role for 15-LOX products of both ω-6 and ω-3 origin in allergic inflammation. Prominent differences at baseline levels indicate that non-symptomatic asthmatics are subject to an underlying inflammatory condition not observed with other traditional mediators. Results suggest that oxylipin profiling may provide a sensitive means of characterizing low-level inflammation and that even individuals with mild disease display distinct phenotypic profiles, which may have clinical ramifications for disease.

Original languageEnglish (US)
Article numbere33780
JournalPLoS One
Volume7
Issue number3
DOIs
StatePublished - Mar 15 2012

Fingerprint

Oxylipins
Betula
oxylipins
Pollen
allergens
Allergens
pollen
Lipids
lipids
lipoxygenase
inflammation
Arachidonate 15-Lipoxygenase
Inflammation
asthma
cytochrome P-450
Respiratory System
Cytochrome P-450 Enzyme System
Asthma
Respiratory system
leukotrienes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lundström, S. L., Yang, J., Källberg, H. J., Thunberg, S., Gafvelin, G., Haeggström, J. Z., ... Wheelock, C. E. (2012). Allergic asthmatics show divergent lipid mediator profiles from healthy controls both at baseline and following birch pollen provocation. PLoS One, 7(3), [e33780]. https://doi.org/10.1371/journal.pone.0033780

Allergic asthmatics show divergent lipid mediator profiles from healthy controls both at baseline and following birch pollen provocation. / Lundström, Susanna L.; Yang, Jun; Källberg, Henrik J.; Thunberg, Sarah; Gafvelin, Guro; Haeggström, Jesper Z.; Grönneberg, Reidar; Grunewald, Johan; van Hage, Marianne; Hammock, Bruce D.; Eklund, Anders; Wheelock, Åsa M.; Wheelock, Craig E.

In: PLoS One, Vol. 7, No. 3, e33780, 15.03.2012.

Research output: Contribution to journalArticle

Lundström, SL, Yang, J, Källberg, HJ, Thunberg, S, Gafvelin, G, Haeggström, JZ, Grönneberg, R, Grunewald, J, van Hage, M, Hammock, BD, Eklund, A, Wheelock, ÅM & Wheelock, CE 2012, 'Allergic asthmatics show divergent lipid mediator profiles from healthy controls both at baseline and following birch pollen provocation', PLoS One, vol. 7, no. 3, e33780. https://doi.org/10.1371/journal.pone.0033780
Lundström, Susanna L. ; Yang, Jun ; Källberg, Henrik J. ; Thunberg, Sarah ; Gafvelin, Guro ; Haeggström, Jesper Z. ; Grönneberg, Reidar ; Grunewald, Johan ; van Hage, Marianne ; Hammock, Bruce D. ; Eklund, Anders ; Wheelock, Åsa M. ; Wheelock, Craig E. / Allergic asthmatics show divergent lipid mediator profiles from healthy controls both at baseline and following birch pollen provocation. In: PLoS One. 2012 ; Vol. 7, No. 3.
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abstract = "Background: Asthma is a respiratory tract disorder characterized by airway hyper-reactivity and chronic inflammation. Allergic asthma is associated with the production of allergen-specific IgE and expansion of allergen-specific T-cell populations. Progression of allergic inflammation is driven by T-helper type 2 (Th2) mediators and is associated with alterations in the levels of lipid mediators. Objectives: Responses of the respiratory system to birch allergen provocation in allergic asthmatics were investigated. Eicosanoids and other oxylipins were quantified in the bronchoalveolar lumen to provide a measure of shifts in lipid mediators associated with allergen challenge in allergic asthmatics. Methods: Eighty-seven lipid mediators representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS following off-line extraction of bronchoalveolar lavage fluid (BALF). Multivariate statistics using OPLS were employed to interrogate acquired oxylipin data in combination with immunological markers. Results: Thirty-two oxylipins were quantified, with baseline asthmatics possessing a different oxylipin profile relative to healthy individuals that became more distinct following allergen provocation. The most prominent differences included 15-LOX-derived ω-3 and ω-6 oxylipins. Shared-and-Unique-Structures (SUS)-plot modeling showed a correlation (R 2 = 0.7) between OPLS models for baseline asthmatics (R 2Y[cum] = 0.87, Q 2[cum] = 0.51) and allergen-provoked asthmatics (R 2Y[cum] = 0.95, Q 2[cum] = 0.73), with the majority of quantified lipid mediators and cytokines contributing equally to both groups. Unique structures for allergen provocation included leukotrienes (LTB 4 and 6-trans-LTB 4), CYP-derivatives of linoleic acid (epoxides/diols), and IL-10. Conclusions: Differences in asthmatic relative to healthy profiles suggest a role for 15-LOX products of both ω-6 and ω-3 origin in allergic inflammation. Prominent differences at baseline levels indicate that non-symptomatic asthmatics are subject to an underlying inflammatory condition not observed with other traditional mediators. Results suggest that oxylipin profiling may provide a sensitive means of characterizing low-level inflammation and that even individuals with mild disease display distinct phenotypic profiles, which may have clinical ramifications for disease.",
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AU - Thunberg, Sarah

AU - Gafvelin, Guro

AU - Haeggström, Jesper Z.

AU - Grönneberg, Reidar

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AU - van Hage, Marianne

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N2 - Background: Asthma is a respiratory tract disorder characterized by airway hyper-reactivity and chronic inflammation. Allergic asthma is associated with the production of allergen-specific IgE and expansion of allergen-specific T-cell populations. Progression of allergic inflammation is driven by T-helper type 2 (Th2) mediators and is associated with alterations in the levels of lipid mediators. Objectives: Responses of the respiratory system to birch allergen provocation in allergic asthmatics were investigated. Eicosanoids and other oxylipins were quantified in the bronchoalveolar lumen to provide a measure of shifts in lipid mediators associated with allergen challenge in allergic asthmatics. Methods: Eighty-seven lipid mediators representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS following off-line extraction of bronchoalveolar lavage fluid (BALF). Multivariate statistics using OPLS were employed to interrogate acquired oxylipin data in combination with immunological markers. Results: Thirty-two oxylipins were quantified, with baseline asthmatics possessing a different oxylipin profile relative to healthy individuals that became more distinct following allergen provocation. The most prominent differences included 15-LOX-derived ω-3 and ω-6 oxylipins. Shared-and-Unique-Structures (SUS)-plot modeling showed a correlation (R 2 = 0.7) between OPLS models for baseline asthmatics (R 2Y[cum] = 0.87, Q 2[cum] = 0.51) and allergen-provoked asthmatics (R 2Y[cum] = 0.95, Q 2[cum] = 0.73), with the majority of quantified lipid mediators and cytokines contributing equally to both groups. Unique structures for allergen provocation included leukotrienes (LTB 4 and 6-trans-LTB 4), CYP-derivatives of linoleic acid (epoxides/diols), and IL-10. Conclusions: Differences in asthmatic relative to healthy profiles suggest a role for 15-LOX products of both ω-6 and ω-3 origin in allergic inflammation. Prominent differences at baseline levels indicate that non-symptomatic asthmatics are subject to an underlying inflammatory condition not observed with other traditional mediators. Results suggest that oxylipin profiling may provide a sensitive means of characterizing low-level inflammation and that even individuals with mild disease display distinct phenotypic profiles, which may have clinical ramifications for disease.

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