Allergen-specific IgG and IgA in serum and bronchoalveolar lavage fluid in a model of experimental feline asthma

C. R. Norris, J. R. Byerly, K. C. Decile, R. D. Berghaus, W. F. Walby, Edward S Schelegle, D. M. Hyde, Laurel J Gershwin

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Allergic asthma, a Th2 cell driven response to inhaled allergens, has classically been thought of as predominantly mediated by IgE antibodies. To investigate the role of other immunoglobulin classes (e.g., IgG and IgA) in the immunopathogenesis of allergic asthma, levels of these allergen-specific immunoglobulins were measured in serum and mucosal fluids. Bermuda grass allergen (BGA)-specific IgG and IgA ELISAs in serum and bronchoalveolar lavage fluid (BALF) were developed and optimized in an experimental model of BGA-induced feline asthma. Levels of BGA-specific IgG and IgA significantly increased over time in serum and BALF after allergen sensitization. Additionally, these elevated levels of BGA-specific IgG and IgA were seen in conjunction with the development of an asthmatic phenotype indicated by positive intradermal skin tests, enhanced airways hyperreactivity, and increased eosinophil percentages in the BALF.

Original languageEnglish (US)
Pages (from-to)119-127
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume96
Issue number3-4
DOIs
StatePublished - Dec 15 2003

Keywords

  • Allergy
  • Antibody
  • Cat
  • ELISA
  • Immunoglobulin
  • Pulmonary

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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