Alginate hydrogels of varied molecular weight distribution enable sustained release of sphingosine-1-phosphate and promote angiogenesis

Priscilla A. Williams, Kevin T. Campbell, Eduardo Silva

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Alginate hydrogels have been widely validated for controlled release of growth factors and cytokines, but studies exploring sustained release of small hydrophobic lipids are lacking. Sphingosine-1-phosphate (S1P), a bioactive lipid, is an appealing small molecule for inducing blood vessel formation in the context of ischemic conditions. However, there are numerous biological and engineering challenges associated with designing biomaterial systems for controlled release of this lipid. Thus, the objective of this study was to design an injectable, alginate hydrogel formulation that provides controlled release of S1P to establish locally sustained concentration gradients that promote neovascularization. Herein, we varied the molecular weight distribution of alginate polymers within the hydrogel to alter the resultant mechanical properties in a manner that provides control over S1P release. With increasing high molecular weight (HMW) content, the hydrogels exhibited stiffer material properties and released S1P at slower rates. Accordingly, S1P released from hydrogels with 100% HMW content led to enhanced directed migration of outgrowth endothelial cells and blood vessel development assessed using a chick chorioallantoic membrane assay as compared to hydrogels with less HMW content. Overall, this study describes how alginate hydrogels of varied molecular weight may be used to control S1P release kinetics for therapeutic applications.

Original languageEnglish (US)
Pages (from-to)138-146
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume106
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Sphingosines
Hydrogels
Alginate
Molecular weight distribution
Phosphates
Molecular weight
Lipids
Hydrogel
Blood vessels
Endothelial cells
Biocompatible Materials
alginic acid
sphingosine 1-phosphate
Assays
Materials properties
Intercellular Signaling Peptides and Proteins
Biomaterials
Polymers
Cytokines
Membranes

Keywords

  • chick chorioallantoic membrane assay
  • endothelial cell migration
  • lipid delivery
  • neovascularization
  • polymeric biomaterials

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Alginate hydrogels of varied molecular weight distribution enable sustained release of sphingosine-1-phosphate and promote angiogenesis. / Williams, Priscilla A.; Campbell, Kevin T.; Silva, Eduardo.

In: Journal of Biomedical Materials Research - Part A, Vol. 106, No. 1, 01.01.2018, p. 138-146.

Research output: Contribution to journalArticle

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