Alginate and DNA gels are suitable delivery systems for diabetic wound healing

Ana Tellechea, Eduardo Silva, Jianghong Min, Ermelindo C. Leal, Michael E. Auster, Leena Pradhan-Nabzdyk, William Shih, David J. Mooney, Aristidis Veves

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Diabetic foot ulcers (DFU) represent a severe health problem and an unmet clinical challenge. In this study, we tested the efficacy of novel biomaterials in improving wound healing in mouse models of diabetes mellitus (DM). The biomaterials are composed of alginate- and deoxyribonucleic acid (DNA)-based gels that allow incorporation of effector cells, such as outgrowth endothelial cells (OEC), and provide sustained release of bioactive factors, such as neuropeptides and growth factors, which have been previously validated in experimental models of DM wound healing or hind limb ischemia. We tested these biomaterials in mice and demonstrate that they are biocompatible and can be injected into the wound margins without major adverse effects. In addition, we show that the combination of OEC and the neuropeptide Substance P has a better healing outcome than the delivery of OEC alone, while subtherapeutic doses of vascular endothelial growth factor (VEGF) are required for the transplanted cells to exert their beneficial effects in wound healing. In summary, alginate and DNA scaffolds could serve as potential delivery systems for the next-generation DFU therapies.

Original languageEnglish (US)
Pages (from-to)146-153
Number of pages8
JournalInternational Journal of Lower Extremity Wounds
Volume14
Issue number2
DOIs
StatePublished - Jan 1 2015

Fingerprint

Biocompatible Materials
Wound Healing
Diabetic Foot
Endothelial Cells
Gels
Neuropeptides
DNA
Experimental Diabetes Mellitus
Substance P
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Diabetes Mellitus
Theoretical Models
Ischemia
Extremities
Health
Wounds and Injuries
alginic acid
Therapeutics

Keywords

  • biomaterials
  • diabetic foot ulcers
  • endothelial precursor cells
  • neuropeptides
  • wound healing

ASJC Scopus subject areas

  • Surgery

Cite this

Alginate and DNA gels are suitable delivery systems for diabetic wound healing. / Tellechea, Ana; Silva, Eduardo; Min, Jianghong; Leal, Ermelindo C.; Auster, Michael E.; Pradhan-Nabzdyk, Leena; Shih, William; Mooney, David J.; Veves, Aristidis.

In: International Journal of Lower Extremity Wounds, Vol. 14, No. 2, 01.01.2015, p. 146-153.

Research output: Contribution to journalArticle

Tellechea, A, Silva, E, Min, J, Leal, EC, Auster, ME, Pradhan-Nabzdyk, L, Shih, W, Mooney, DJ & Veves, A 2015, 'Alginate and DNA gels are suitable delivery systems for diabetic wound healing', International Journal of Lower Extremity Wounds, vol. 14, no. 2, pp. 146-153. https://doi.org/10.1177/1534734615580018
Tellechea, Ana ; Silva, Eduardo ; Min, Jianghong ; Leal, Ermelindo C. ; Auster, Michael E. ; Pradhan-Nabzdyk, Leena ; Shih, William ; Mooney, David J. ; Veves, Aristidis. / Alginate and DNA gels are suitable delivery systems for diabetic wound healing. In: International Journal of Lower Extremity Wounds. 2015 ; Vol. 14, No. 2. pp. 146-153.
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