Abstract
Diabetic foot ulcers (DFU) represent a severe health problem and an unmet clinical challenge. In this study, we tested the efficacy of novel biomaterials in improving wound healing in mouse models of diabetes mellitus (DM). The biomaterials are composed of alginate- and deoxyribonucleic acid (DNA)-based gels that allow incorporation of effector cells, such as outgrowth endothelial cells (OEC), and provide sustained release of bioactive factors, such as neuropeptides and growth factors, which have been previously validated in experimental models of DM wound healing or hind limb ischemia. We tested these biomaterials in mice and demonstrate that they are biocompatible and can be injected into the wound margins without major adverse effects. In addition, we show that the combination of OEC and the neuropeptide Substance P has a better healing outcome than the delivery of OEC alone, while subtherapeutic doses of vascular endothelial growth factor (VEGF) are required for the transplanted cells to exert their beneficial effects in wound healing. In summary, alginate and DNA scaffolds could serve as potential delivery systems for the next-generation DFU therapies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 146-153 |
| Number of pages | 8 |
| Journal | International Journal of Lower Extremity Wounds |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 2015 |
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Keywords
- biomaterials
- diabetic foot ulcers
- endothelial precursor cells
- neuropeptides
- wound healing
ASJC Scopus subject areas
- Surgery
Cite this
Alginate and DNA gels are suitable delivery systems for diabetic wound healing. / Tellechea, Ana; Silva, Eduardo; Min, Jianghong; Leal, Ermelindo C.; Auster, Michael E.; Pradhan-Nabzdyk, Leena; Shih, William; Mooney, David J.; Veves, Aristidis.
In: International Journal of Lower Extremity Wounds, Vol. 14, No. 2, 01.01.2015, p. 146-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alginate and DNA gels are suitable delivery systems for diabetic wound healing
AU - Tellechea, Ana
AU - Silva, Eduardo
AU - Min, Jianghong
AU - Leal, Ermelindo C.
AU - Auster, Michael E.
AU - Pradhan-Nabzdyk, Leena
AU - Shih, William
AU - Mooney, David J.
AU - Veves, Aristidis
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Diabetic foot ulcers (DFU) represent a severe health problem and an unmet clinical challenge. In this study, we tested the efficacy of novel biomaterials in improving wound healing in mouse models of diabetes mellitus (DM). The biomaterials are composed of alginate- and deoxyribonucleic acid (DNA)-based gels that allow incorporation of effector cells, such as outgrowth endothelial cells (OEC), and provide sustained release of bioactive factors, such as neuropeptides and growth factors, which have been previously validated in experimental models of DM wound healing or hind limb ischemia. We tested these biomaterials in mice and demonstrate that they are biocompatible and can be injected into the wound margins without major adverse effects. In addition, we show that the combination of OEC and the neuropeptide Substance P has a better healing outcome than the delivery of OEC alone, while subtherapeutic doses of vascular endothelial growth factor (VEGF) are required for the transplanted cells to exert their beneficial effects in wound healing. In summary, alginate and DNA scaffolds could serve as potential delivery systems for the next-generation DFU therapies.
AB - Diabetic foot ulcers (DFU) represent a severe health problem and an unmet clinical challenge. In this study, we tested the efficacy of novel biomaterials in improving wound healing in mouse models of diabetes mellitus (DM). The biomaterials are composed of alginate- and deoxyribonucleic acid (DNA)-based gels that allow incorporation of effector cells, such as outgrowth endothelial cells (OEC), and provide sustained release of bioactive factors, such as neuropeptides and growth factors, which have been previously validated in experimental models of DM wound healing or hind limb ischemia. We tested these biomaterials in mice and demonstrate that they are biocompatible and can be injected into the wound margins without major adverse effects. In addition, we show that the combination of OEC and the neuropeptide Substance P has a better healing outcome than the delivery of OEC alone, while subtherapeutic doses of vascular endothelial growth factor (VEGF) are required for the transplanted cells to exert their beneficial effects in wound healing. In summary, alginate and DNA scaffolds could serve as potential delivery systems for the next-generation DFU therapies.
KW - biomaterials
KW - diabetic foot ulcers
KW - endothelial precursor cells
KW - neuropeptides
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=84937551409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937551409&partnerID=8YFLogxK
U2 - 10.1177/1534734615580018
DO - 10.1177/1534734615580018
M3 - Article
C2 - 26032947
AN - SCOPUS:84937551409
VL - 14
SP - 146
EP - 153
JO - International Journal of Lower Extremity Wounds
JF - International Journal of Lower Extremity Wounds
SN - 1534-7346
IS - 2
ER -