Abstract
The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 726-728 |
| Number of pages | 3 |
| Journal | NeuroReport |
| Volume | 7 |
| Issue number | 3 |
| State | Published - 1996 |
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Keywords
- F
- Aldose reductase
- Diabetes mellitus
- NMR
- Sorbitol dehydrogenase
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Aldose reductase and sorbitol dehydrogenase activities in diabetic brain : In vivo kinetic studies using 19F 3-FDG NMR in rats. / Kwee, Ingrid L.; Igarashi, Hironaka; Nakada, Tsutomu.
In: NeuroReport, Vol. 7, No. 3, 1996, p. 726-728.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aldose reductase and sorbitol dehydrogenase activities in diabetic brain
T2 - In vivo kinetic studies using 19F 3-FDG NMR in rats
AU - Kwee, Ingrid L.
AU - Igarashi, Hironaka
AU - Nakada, Tsutomu
PY - 1996
Y1 - 1996
N2 - The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.
AB - The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.
KW - F
KW - Aldose reductase
KW - Diabetes mellitus
KW - NMR
KW - Sorbitol dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0030003540&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030003540&partnerID=8YFLogxK
M3 - Article
C2 - 8733731
AN - SCOPUS:0030003540
VL - 7
SP - 726
EP - 728
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 3
ER -