Aldose reductase and sorbitol dehydrogenase activities in diabetic brain: In vivo kinetic studies using 19F 3-FDG NMR in rats

Ingrid L. Kwee, Hironaka Igarashi, Tsutomu Nakada

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.

Original languageEnglish (US)
Pages (from-to)726-728
Number of pages3
JournalNeuroReport
Volume7
Issue number3
StatePublished - 1996

Keywords

  • F
  • Aldose reductase
  • Diabetes mellitus
  • NMR
  • Sorbitol dehydrogenase

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Aldose reductase and sorbitol dehydrogenase activities in diabetic brain: In vivo kinetic studies using <sup>19</sup>F 3-FDG NMR in rats'. Together they form a unique fingerprint.

  • Cite this