Aldose reductase and sorbitol dehydrogenase activities in diabetic brain

In vivo kinetic studies using 19F 3-FDG NMR in rats

Ingrid Kwee, Hironaka Igarashi, Tsutomu Nakada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.

Original languageEnglish (US)
Pages (from-to)726-728
Number of pages3
JournalNeuroReport
Volume7
Issue number3
StatePublished - 1996

Fingerprint

L-Iditol 2-Dehydrogenase
Aldehyde Reductase
Fluorine
Sorbitol
Magnetic Resonance Spectroscopy
Fructose
Brain
Diabetes Complications
Diabetes Mellitus
3-deoxy-3-fluoro-D-glucose

Keywords

  • F
  • Aldose reductase
  • Diabetes mellitus
  • NMR
  • Sorbitol dehydrogenase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Aldose reductase and sorbitol dehydrogenase activities in diabetic brain : In vivo kinetic studies using 19F 3-FDG NMR in rats. / Kwee, Ingrid; Igarashi, Hironaka; Nakada, Tsutomu.

In: NeuroReport, Vol. 7, No. 3, 1996, p. 726-728.

Research output: Contribution to journalArticle

@article{0caff924a6e64100a270aafea964a699,
title = "Aldose reductase and sorbitol dehydrogenase activities in diabetic brain: In vivo kinetic studies using 19F 3-FDG NMR in rats",
abstract = "The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.",
keywords = "F, Aldose reductase, Diabetes mellitus, NMR, Sorbitol dehydrogenase",
author = "Ingrid Kwee and Hironaka Igarashi and Tsutomu Nakada",
year = "1996",
language = "English (US)",
volume = "7",
pages = "726--728",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Aldose reductase and sorbitol dehydrogenase activities in diabetic brain

T2 - In vivo kinetic studies using 19F 3-FDG NMR in rats

AU - Kwee, Ingrid

AU - Igarashi, Hironaka

AU - Nakada, Tsutomu

PY - 1996

Y1 - 1996

N2 - The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.

AB - The effects of diabetes mellitus on the kinetic constants of aldose reductase and sorbitol dehydrogenase in rat brain were investigated non-invasively in vivo using the 3-fluoro-3-deoxy-D-glucose (3-FDG) 19-fluorine (19F) nuclear magnetic resonance (NMR) method. While forward flux for both aldose reductase and sorbitol dehydrogen-ase (k1 and k2) were significantly increased, there was no corresponding increase in reverse flux (k3 and k4), and leakage of fructose (k5) was negligible. These findings indicate that the enzymatic kinetics of aldose reductase sorbitol (ARS) in diabetic brain undergo alteration favoring intracellular sorbitol and fructose accumulation, the frequently implicated biochemical basis of diabetic complications.

KW - F

KW - Aldose reductase

KW - Diabetes mellitus

KW - NMR

KW - Sorbitol dehydrogenase

UR - http://www.scopus.com/inward/record.url?scp=0030003540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030003540&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 726

EP - 728

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 3

ER -