Abstract
Background: The objective of this study is to assess if there were any changes in liver biopsies after treatment with S-adenosyl-. l-methionine(SAMe) in alcoholic liver disease patients. Methods: Liver biopsies of 14 patients were randomized for SAMe treatment at week 0 (biopsy #1) and at 24. weeks (biopsy #2). Patients received 1.2. g of SAMe or placebo by mouth daily and stopped alcohol intake. Biopsies were semi-quantitatively scored for: steatosis, inflammation, necrosis, fibrosis, apoptosis by TUNEL stain, percent fibrosis per square field, smooth muscle actin stain, Kupffer cells, polymorphonuclear leukocytes, lipogranules, lymphocytes, balloon cell formation, Mallory-Denk bodies, and duct metaplasia. Results: Comparing treatment arm to placebo arm, no significant difference was found between biopsy #1 and biopsy #2. However, when both study arms were grouped together, there was decrease in smooth muscle actin stain, where the P-value. =. 0.027. Conclusion: Treatment with SAMe did not show a statistically significant difference in the characteristics studied. However, when both the treatment and placebo arm data were grouped together to increase the n and power, there was a decrease in the smooth muscle actin stain, reflecting a decrease in stellate cells activation, likely due to the alcohol abstinence. This study suggests that it may not be beneficial to wait for more definitive treatment, like liver transplant in alcoholic liver disease patients, since the liver tissue remained largely with the same degree of pathology six months out, regardless of treatment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 187-191 |
| Number of pages | 5 |
| Journal | Experimental and Molecular Pathology |
| Volume | 95 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 2013 |
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Keywords
- Alcoholic liver disease
- Liver biopsies
- S-adenosyl-l-methionine
- Smooth muscle actin
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Pathology and Forensic Medicine
Cite this
Alcoholic liver disease patients treated with S-adenosyl-l-methionine : An in-depth look at liver morphologic data comparing pre and post treatment liver biopsies. / Le, Mary D.; Enbom, Elena; Traum, Peter K.; Medici, Valentina; Halsted, Charles H.; French, Samuel W.
In: Experimental and Molecular Pathology, Vol. 95, No. 2, 10.2013, p. 187-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alcoholic liver disease patients treated with S-adenosyl-l-methionine
T2 - An in-depth look at liver morphologic data comparing pre and post treatment liver biopsies
AU - Le, Mary D.
AU - Enbom, Elena
AU - Traum, Peter K.
AU - Medici, Valentina
AU - Halsted, Charles H.
AU - French, Samuel W.
PY - 2013/10
Y1 - 2013/10
N2 - Background: The objective of this study is to assess if there were any changes in liver biopsies after treatment with S-adenosyl-. l-methionine(SAMe) in alcoholic liver disease patients. Methods: Liver biopsies of 14 patients were randomized for SAMe treatment at week 0 (biopsy #1) and at 24. weeks (biopsy #2). Patients received 1.2. g of SAMe or placebo by mouth daily and stopped alcohol intake. Biopsies were semi-quantitatively scored for: steatosis, inflammation, necrosis, fibrosis, apoptosis by TUNEL stain, percent fibrosis per square field, smooth muscle actin stain, Kupffer cells, polymorphonuclear leukocytes, lipogranules, lymphocytes, balloon cell formation, Mallory-Denk bodies, and duct metaplasia. Results: Comparing treatment arm to placebo arm, no significant difference was found between biopsy #1 and biopsy #2. However, when both study arms were grouped together, there was decrease in smooth muscle actin stain, where the P-value. =. 0.027. Conclusion: Treatment with SAMe did not show a statistically significant difference in the characteristics studied. However, when both the treatment and placebo arm data were grouped together to increase the n and power, there was a decrease in the smooth muscle actin stain, reflecting a decrease in stellate cells activation, likely due to the alcohol abstinence. This study suggests that it may not be beneficial to wait for more definitive treatment, like liver transplant in alcoholic liver disease patients, since the liver tissue remained largely with the same degree of pathology six months out, regardless of treatment.
AB - Background: The objective of this study is to assess if there were any changes in liver biopsies after treatment with S-adenosyl-. l-methionine(SAMe) in alcoholic liver disease patients. Methods: Liver biopsies of 14 patients were randomized for SAMe treatment at week 0 (biopsy #1) and at 24. weeks (biopsy #2). Patients received 1.2. g of SAMe or placebo by mouth daily and stopped alcohol intake. Biopsies were semi-quantitatively scored for: steatosis, inflammation, necrosis, fibrosis, apoptosis by TUNEL stain, percent fibrosis per square field, smooth muscle actin stain, Kupffer cells, polymorphonuclear leukocytes, lipogranules, lymphocytes, balloon cell formation, Mallory-Denk bodies, and duct metaplasia. Results: Comparing treatment arm to placebo arm, no significant difference was found between biopsy #1 and biopsy #2. However, when both study arms were grouped together, there was decrease in smooth muscle actin stain, where the P-value. =. 0.027. Conclusion: Treatment with SAMe did not show a statistically significant difference in the characteristics studied. However, when both the treatment and placebo arm data were grouped together to increase the n and power, there was a decrease in the smooth muscle actin stain, reflecting a decrease in stellate cells activation, likely due to the alcohol abstinence. This study suggests that it may not be beneficial to wait for more definitive treatment, like liver transplant in alcoholic liver disease patients, since the liver tissue remained largely with the same degree of pathology six months out, regardless of treatment.
KW - Alcoholic liver disease
KW - Liver biopsies
KW - S-adenosyl-l-methionine
KW - Smooth muscle actin
UR - http://www.scopus.com/inward/record.url?scp=84882807358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882807358&partnerID=8YFLogxK
U2 - 10.1016/j.yexmp.2013.07.003
DO - 10.1016/j.yexmp.2013.07.003
M3 - Article
C2 - 23886644
AN - SCOPUS:84882807358
VL - 95
SP - 187
EP - 191
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
SN - 0014-4800
IS - 2
ER -