Albumin modulation of capillary permeability: Test of an adsorption mechanism

V. H. Huxley, F. E. Curry

Research output: Contribution to journalArticle

Abstract

We investigated further the mechanism whereby albumin interacts with the walls of capillary blood vessels to maintain normal permeability properties. In individually perfused capillaries of frog mesentery, hydraulic conductivity was measured as the albumin perfusate concentration was first reduced in three steps from 0.1 to 0 g/dl (intermediate concns 0.01 and 0.001 g/dl), then increased in up to four steps to 1 g/dl (intermediate concns 0.001, 0.01, and 0.1 g/dl). The albumin concentration required to return hydraulic conductivity toward control values, following Ringer perfusion, was at least an order of magnitude larger than that required to maintain permeability close to control values prior to Ringer perfusion. The experiments indicate that the affinity of albumin for binding sites on the capillary wall following Ringer perfusion is less than the albumin affinity for binding sites after the capillary has been perfused with solutions containing 0.1 g/dl albumin. Before the hysteresis of hydraulic conductivity on albumin concentration can be understood, the factors determining the transport of albumin into the membrane must be investigated further.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume17
Issue number2
StatePublished - 1985

Fingerprint

Capillary Permeability
Adsorption
Albumins
Perfusion
Permeability
Binding Sites
Mesentery
Anura
Blood Vessels
Membranes

ASJC Scopus subject areas

  • Physiology

Cite this

Albumin modulation of capillary permeability : Test of an adsorption mechanism. / Huxley, V. H.; Curry, F. E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 17, No. 2, 1985.

Research output: Contribution to journalArticle

@article{76a028f300f94952a9e0202e6ddc3705,
title = "Albumin modulation of capillary permeability: Test of an adsorption mechanism",
abstract = "We investigated further the mechanism whereby albumin interacts with the walls of capillary blood vessels to maintain normal permeability properties. In individually perfused capillaries of frog mesentery, hydraulic conductivity was measured as the albumin perfusate concentration was first reduced in three steps from 0.1 to 0 g/dl (intermediate concns 0.01 and 0.001 g/dl), then increased in up to four steps to 1 g/dl (intermediate concns 0.001, 0.01, and 0.1 g/dl). The albumin concentration required to return hydraulic conductivity toward control values, following Ringer perfusion, was at least an order of magnitude larger than that required to maintain permeability close to control values prior to Ringer perfusion. The experiments indicate that the affinity of albumin for binding sites on the capillary wall following Ringer perfusion is less than the albumin affinity for binding sites after the capillary has been perfused with solutions containing 0.1 g/dl albumin. Before the hysteresis of hydraulic conductivity on albumin concentration can be understood, the factors determining the transport of albumin into the membrane must be investigated further.",
author = "Huxley, {V. H.} and Curry, {F. E.}",
year = "1985",
language = "English (US)",
volume = "17",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Albumin modulation of capillary permeability

T2 - Test of an adsorption mechanism

AU - Huxley, V. H.

AU - Curry, F. E.

PY - 1985

Y1 - 1985

N2 - We investigated further the mechanism whereby albumin interacts with the walls of capillary blood vessels to maintain normal permeability properties. In individually perfused capillaries of frog mesentery, hydraulic conductivity was measured as the albumin perfusate concentration was first reduced in three steps from 0.1 to 0 g/dl (intermediate concns 0.01 and 0.001 g/dl), then increased in up to four steps to 1 g/dl (intermediate concns 0.001, 0.01, and 0.1 g/dl). The albumin concentration required to return hydraulic conductivity toward control values, following Ringer perfusion, was at least an order of magnitude larger than that required to maintain permeability close to control values prior to Ringer perfusion. The experiments indicate that the affinity of albumin for binding sites on the capillary wall following Ringer perfusion is less than the albumin affinity for binding sites after the capillary has been perfused with solutions containing 0.1 g/dl albumin. Before the hysteresis of hydraulic conductivity on albumin concentration can be understood, the factors determining the transport of albumin into the membrane must be investigated further.

AB - We investigated further the mechanism whereby albumin interacts with the walls of capillary blood vessels to maintain normal permeability properties. In individually perfused capillaries of frog mesentery, hydraulic conductivity was measured as the albumin perfusate concentration was first reduced in three steps from 0.1 to 0 g/dl (intermediate concns 0.01 and 0.001 g/dl), then increased in up to four steps to 1 g/dl (intermediate concns 0.001, 0.01, and 0.1 g/dl). The albumin concentration required to return hydraulic conductivity toward control values, following Ringer perfusion, was at least an order of magnitude larger than that required to maintain permeability close to control values prior to Ringer perfusion. The experiments indicate that the affinity of albumin for binding sites on the capillary wall following Ringer perfusion is less than the albumin affinity for binding sites after the capillary has been perfused with solutions containing 0.1 g/dl albumin. Before the hysteresis of hydraulic conductivity on albumin concentration can be understood, the factors determining the transport of albumin into the membrane must be investigated further.

UR - http://www.scopus.com/inward/record.url?scp=0021993629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021993629&partnerID=8YFLogxK

M3 - Article

VL - 17

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 2

ER -