Albumin and transferrin synthesis are increased in H4 cells by serum from analbuminemic or nephrotic rats

Xihua Sun, George Kaysen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The hepatic synthesis of several proteins, including both albumin and transferrin is increased in the nephrotic syndrome. While active suppression of albumin synthesis by lymphokines has been described, it has been assumed that augmentation of albumin synthesis is governed by a physical factor, plasma oncotic pressure (π), and that this regulation is by a direct effect of π on hepatocytes. The mechanisms have not been defined. Furthermore, experiments relying on suppression of protein synthesis may only test non-specific inhibitory effects of the experimental intervention. We tested an alternative hypothesis that a serum factor(s) present in hypooncotic states stimulates albumin synthesis. We incubated an immortalized cell line derived from rat hepatocytes (H4 cells) with serum from Nagase analbuminemic rats (NAR) and rats with passive Heymann nephritis (HN), a model of the nephrotic syndrome. Synthesis (incorporation of [35S]methionine) into both albumin and transferrin was increased significantly. The stimulatory effect of these sera was not extinguished by addition of rat or human albumin to the medium prior to or during incubation, even when π in the incubation medium was increased to normal plasma levels by added albumin. Incorporation of [35S]methionine into albumin was 7841 ± 394 cpm/mg cell protein using 10% NAR serum in the presence of human albumin (medium π 26.1 ± 0.17) versus 5149 ± 420 cpm incorporation (P < 0.05) in the presence of control serum and in the absence of added albumin (medium π 2.06 ± 0.26 mm Hg, P < 0.001). The stimulatory activity was preserved following heating of serum for one hour at 60°C. These observations suggest that albumin and transferrin synthesis is stimulated by a heat stable factor found in serum when plasma π is reduced, rather than as a consequence of the interaction of π directly with the hepatocyte.

Original languageEnglish (US)
Pages (from-to)1381-1387
Number of pages7
JournalKidney International
Volume45
Issue number5
StatePublished - May 1994

Fingerprint

Transferrin
Albumins
Serum
Hepatocytes
Acetylglucosaminidase
Nephrotic Syndrome
Methionine
Membranous Glomerulonephritis
Proteins
Lymphokines
Heating
Hot Temperature
Pressure
Cell Line

ASJC Scopus subject areas

  • Nephrology

Cite this

Albumin and transferrin synthesis are increased in H4 cells by serum from analbuminemic or nephrotic rats. / Sun, Xihua; Kaysen, George.

In: Kidney International, Vol. 45, No. 5, 05.1994, p. 1381-1387.

Research output: Contribution to journalArticle

@article{0960c8267cf14cbca62577fd145261d7,
title = "Albumin and transferrin synthesis are increased in H4 cells by serum from analbuminemic or nephrotic rats",
abstract = "The hepatic synthesis of several proteins, including both albumin and transferrin is increased in the nephrotic syndrome. While active suppression of albumin synthesis by lymphokines has been described, it has been assumed that augmentation of albumin synthesis is governed by a physical factor, plasma oncotic pressure (π), and that this regulation is by a direct effect of π on hepatocytes. The mechanisms have not been defined. Furthermore, experiments relying on suppression of protein synthesis may only test non-specific inhibitory effects of the experimental intervention. We tested an alternative hypothesis that a serum factor(s) present in hypooncotic states stimulates albumin synthesis. We incubated an immortalized cell line derived from rat hepatocytes (H4 cells) with serum from Nagase analbuminemic rats (NAR) and rats with passive Heymann nephritis (HN), a model of the nephrotic syndrome. Synthesis (incorporation of [35S]methionine) into both albumin and transferrin was increased significantly. The stimulatory effect of these sera was not extinguished by addition of rat or human albumin to the medium prior to or during incubation, even when π in the incubation medium was increased to normal plasma levels by added albumin. Incorporation of [35S]methionine into albumin was 7841 ± 394 cpm/mg cell protein using 10{\%} NAR serum in the presence of human albumin (medium π 26.1 ± 0.17) versus 5149 ± 420 cpm incorporation (P < 0.05) in the presence of control serum and in the absence of added albumin (medium π 2.06 ± 0.26 mm Hg, P < 0.001). The stimulatory activity was preserved following heating of serum for one hour at 60°C. These observations suggest that albumin and transferrin synthesis is stimulated by a heat stable factor found in serum when plasma π is reduced, rather than as a consequence of the interaction of π directly with the hepatocyte.",
author = "Xihua Sun and George Kaysen",
year = "1994",
month = "5",
language = "English (US)",
volume = "45",
pages = "1381--1387",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Albumin and transferrin synthesis are increased in H4 cells by serum from analbuminemic or nephrotic rats

AU - Sun, Xihua

AU - Kaysen, George

PY - 1994/5

Y1 - 1994/5

N2 - The hepatic synthesis of several proteins, including both albumin and transferrin is increased in the nephrotic syndrome. While active suppression of albumin synthesis by lymphokines has been described, it has been assumed that augmentation of albumin synthesis is governed by a physical factor, plasma oncotic pressure (π), and that this regulation is by a direct effect of π on hepatocytes. The mechanisms have not been defined. Furthermore, experiments relying on suppression of protein synthesis may only test non-specific inhibitory effects of the experimental intervention. We tested an alternative hypothesis that a serum factor(s) present in hypooncotic states stimulates albumin synthesis. We incubated an immortalized cell line derived from rat hepatocytes (H4 cells) with serum from Nagase analbuminemic rats (NAR) and rats with passive Heymann nephritis (HN), a model of the nephrotic syndrome. Synthesis (incorporation of [35S]methionine) into both albumin and transferrin was increased significantly. The stimulatory effect of these sera was not extinguished by addition of rat or human albumin to the medium prior to or during incubation, even when π in the incubation medium was increased to normal plasma levels by added albumin. Incorporation of [35S]methionine into albumin was 7841 ± 394 cpm/mg cell protein using 10% NAR serum in the presence of human albumin (medium π 26.1 ± 0.17) versus 5149 ± 420 cpm incorporation (P < 0.05) in the presence of control serum and in the absence of added albumin (medium π 2.06 ± 0.26 mm Hg, P < 0.001). The stimulatory activity was preserved following heating of serum for one hour at 60°C. These observations suggest that albumin and transferrin synthesis is stimulated by a heat stable factor found in serum when plasma π is reduced, rather than as a consequence of the interaction of π directly with the hepatocyte.

AB - The hepatic synthesis of several proteins, including both albumin and transferrin is increased in the nephrotic syndrome. While active suppression of albumin synthesis by lymphokines has been described, it has been assumed that augmentation of albumin synthesis is governed by a physical factor, plasma oncotic pressure (π), and that this regulation is by a direct effect of π on hepatocytes. The mechanisms have not been defined. Furthermore, experiments relying on suppression of protein synthesis may only test non-specific inhibitory effects of the experimental intervention. We tested an alternative hypothesis that a serum factor(s) present in hypooncotic states stimulates albumin synthesis. We incubated an immortalized cell line derived from rat hepatocytes (H4 cells) with serum from Nagase analbuminemic rats (NAR) and rats with passive Heymann nephritis (HN), a model of the nephrotic syndrome. Synthesis (incorporation of [35S]methionine) into both albumin and transferrin was increased significantly. The stimulatory effect of these sera was not extinguished by addition of rat or human albumin to the medium prior to or during incubation, even when π in the incubation medium was increased to normal plasma levels by added albumin. Incorporation of [35S]methionine into albumin was 7841 ± 394 cpm/mg cell protein using 10% NAR serum in the presence of human albumin (medium π 26.1 ± 0.17) versus 5149 ± 420 cpm incorporation (P < 0.05) in the presence of control serum and in the absence of added albumin (medium π 2.06 ± 0.26 mm Hg, P < 0.001). The stimulatory activity was preserved following heating of serum for one hour at 60°C. These observations suggest that albumin and transferrin synthesis is stimulated by a heat stable factor found in serum when plasma π is reduced, rather than as a consequence of the interaction of π directly with the hepatocyte.

UR - http://www.scopus.com/inward/record.url?scp=0028354449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028354449&partnerID=8YFLogxK

M3 - Article

C2 - 7520954

AN - SCOPUS:0028354449

VL - 45

SP - 1381

EP - 1387

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 5

ER -