AKAP150-anchored PKA activity is important for LTD during its induction phase

Yuan Lu, Mingxu Zhang, Indra A. Lim, Duane D. Hall, Margaret Allen, Yuliya Medvedeva, G. Stanley McKnight, Yuriy M. Usachev, Johannes W Hell

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Protein kinase A (PKA) is thought to tonically maintain an enhanced level of postsynaptic AMPA receptor responses. Injection of PKA inhibitory peptides leads to a run-down of AMPA receptor responses and prevents long-term depression (LTD). This run-down of AMPA receptor activity was proposed to occlude a further reduction that would otherwise constitute LTD. PKA is recruited to postsynaptic sites by the A kinase anchor protein AKAP150. We found that LTD was strongly impaired in acute hippocampal slices from 2-week-old mice in which the PKA binding site on AKAP150 had been genetically deleted (D36 mice). However, basal postsynaptic AMPA and NMDA receptor activity was indistinguishable between D36 and WT mice. During extracellular recordings of field EPSPs and during intracellular recording of EPSCs from hippocampal slices from WT mice, H-89 and KT5720, two structurally different PKA inhibitors, inhibited LTD by more than 70% without affecting basal synaptic transmission or basal phosphorylation of serine 845 on GluR1. Collectively our data indicate that AKAP150-anchored PKA activity is required to induce LTD and not merely to maintain a tonically heightened activity level of AMPA receptors as proposed earlier.

Original languageEnglish (US)
Pages (from-to)4155-4164
Number of pages10
JournalJournal of Physiology
Volume586
Issue number17
DOIs
StatePublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Physiology

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    Lu, Y., Zhang, M., Lim, I. A., Hall, D. D., Allen, M., Medvedeva, Y., McKnight, G. S., Usachev, Y. M., & Hell, J. W. (2008). AKAP150-anchored PKA activity is important for LTD during its induction phase. Journal of Physiology, 586(17), 4155-4164. https://doi.org/10.1113/jphysiol.2008.151662