Airway transcriptomic profiling after bronchial thermoplasty

Shu Yi Liao, Angela L. Linderholm, Ken Y. Yoneda, Nicholas J. Kenyon, Richart W. Harper

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Background: Bronchial thermoplasty is a nonpharmacological, device-based treatment option for a specific population of severe asthmatic subjects, but the underlying mechanisms are largely unknown. The purpose of this study is to identify potential altered pathways by bronchial thermoplasty using a transcriptomic approach. Methods: Patients undergoing bronchial thermoplasty were recruited to the study, and a bronchial brushing sample was obtained before each bronchial thermoplasty session and sent for RNA sequencing. A variance component score test was performed to identify those genes whose expression varied after bronchial thermoplasty sessions. Differential gene expression meta-analysis of severe asthmatic subjects versus controls was performed using public repositories. Overlapping genes were included for downstream pathway and network analyses. Results: 12 patients were enrolled in our study. A total of 133 severe asthma cases and 107 healthy controls from the public repositories were included in the meta-analysis. Comparison of differentially expressed genes from our study patients with the public repositories identified eight overlapping genes: AMIGO2, CBX7, NR3C2, SETBP1, SHANK2, SNTB1, STXBP1 and ZNF853. Network analysis of these overlapping genes identified pathways associated with neurophysiological processes. Conclusion: We have shown that bronchial thermoplasty treatment alters several gene networks that are important in asthma pathogenesis. These results potentially elucidate the disease-modifying mechanisms of bronchial thermoplasty and provide several targets for further investigation.

Original languageEnglish (US)
Article number00123-2018
JournalERJ Open Research
Volume5
Issue number1
DOIs
StatePublished - Feb 1 2019

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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