Airway hyperresponsiveness, elevation of serum-specific IgE and activation of T cells following allergen exposure in sensitized Brown-Norway rats

Angela Franciska Haczku, K. F. Chung, J. Sun, P. J. Barnes, A. B. Kay, R. Moqbel

Research output: Contribution to journalArticle

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Abstract

T lymphocytes may play a regulatory role in the development of allergic airway hyperresponsiveness (AHR). We have studied the relationship between airway responsiveness and a number of immunological changes in Brown-Norway rats sensitized intraperitoneally and repeatedly exposed to ovalbumin (OVA) aerosol. Acetylcholine provocation concentration (PC)150 (the concentration of acetylcholine causing a 150% increase of base-line lung resistance) was measured and peripheral blood and bronchoalveolar lavage (BAL) cells were collected 18-24hr after the final exposure. Total and OVA-specific IgE in serum was measured by enzyme-linked immunosorbent assay (ELISA). Mononuclear cells were analysed by flow cytometry after labelling with monoclonal antibodies against CD2 (pan T-cell marker), CD4, CD8 (T-cell subsets) or CD25 (interleukin-2 receptor). There were significant differences in PC150 (P < 0.05) and in OVA-specific IgE levels in serum (P < 0.002); CD4+ T cells expressed a significantly increased level of CD25 immunoreactivity in BAL, but not in peripheral blood, of rats sensitized and exposed to OVA, compared with saline-exposed controls (P < 0.02). There was a significant correlation between CD25 expression and BAL eosinophil numbers (r = 0.74, P < 0.001), PC150 (r = 0.63, P < 0.003) and OVA-specific IgE (r = 0.77, P < 0.001). These data suggest that activated T cells may be involved in the regulation of allergen-induced AHR in a relevant animal model of allergic asthma.

Original languageEnglish (US)
Pages (from-to)598-603
Number of pages6
JournalImmunology
Volume85
Issue number4
StatePublished - 1995
Externally publishedYes

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Ovalbumin
Allergens
Immunoglobulin E
T-Lymphocytes
Bronchoalveolar Lavage
Serum
Acetylcholine
Interleukin-2 Receptors
T-Lymphocyte Subsets
Aerosols
Eosinophils
Flow Cytometry
Asthma
Animal Models
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Lung

ASJC Scopus subject areas

  • Immunology

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Airway hyperresponsiveness, elevation of serum-specific IgE and activation of T cells following allergen exposure in sensitized Brown-Norway rats. / Haczku, Angela Franciska; Chung, K. F.; Sun, J.; Barnes, P. J.; Kay, A. B.; Moqbel, R.

In: Immunology, Vol. 85, No. 4, 1995, p. 598-603.

Research output: Contribution to journalArticle

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