Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene

Gill Diamond, Douglas E. Jones, Charles L Bevins

Research output: Contribution to journalArticle

133 Scopus citations

Abstract

We previously reported the isolation and characterization of a broad-spectrum antimicrobial peptide from the bovine tracheal mucosa, which we called tracheal antimicrobial peptide (TAP). We now show the TAP gene is expressed throughout the adult conducting airway, from nasal to bronchiolar tissue, but not in tissues other than airway mucosa, as determined by Northern blot analysis. In situ hybridization of airway sections localizes TAP mRNA to columnar cells of the pseudostratified epithelium. We report the structural organization of the TAP gene and show that TAP is a member of a large family of related sequences with high nucleotide identity in the 5′ exon. The data support the hypothesis that antimicrobial peptides contribute to host defense of the respiratory tract.

Original languageEnglish (US)
Pages (from-to)4596-4600
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number10
StatePublished - May 15 1993
Externally publishedYes

Keywords

  • Defensin-like
  • Host defense
  • Respiratory tract

ASJC Scopus subject areas

  • General
  • Genetics

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