AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration

David Li, Chongfei Jin, Xiaodong Jiao, Lin Li, Tahmina Bushra, Muhammad Asif Naeem, Nadeem H. Butt, Tayyab Husnain, Paul A. Sieving, Sheikh Riazuddin, S. Amer Riazuddin, J. Fielding Hejtmancik

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Purpose: To localize and identify the gene and mutations causing autosomal recessive retinal dystrophy in two consanguineous Pakistani families. Methods: Consanguineous families from Pakistan were ascertained to be affected with autosomal recessive retinal degeneration. All affected individuals underwent thorough ophthalmologic examinations. Blood samples were collected, and genomic DNA was extracted using a salting out procedure. Genotyping was performed using microsatellite markers spaced at approximately 10 cM intervals. Two-point linkage analysis was performed with the lod score method. Direct DNA sequencing of amplified genomic DNA was performed for mutation screening of candidate genes. Results: Genome-wide linkage scans yielded a lod score of 3.05 at θ=0 for D17S1832 and 3.82 at θ=0 for D17S938, localizing the disease gene to a 12.22 cM (6.64 Mb) region flanked by D17S1828 and D17S1852 for family 61032 and family 61227, which contains aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), a gene previously implicated in recessive Leber congenital amaurosis and autosomal dominant cone-rod dystrophy. Sequencing of AIPL1 showed a homozygous c.773G>C (p.Arg258Pro) sequence change in all affected individuals of family 61032 and a homozygous c.465G>T (p.(H93_Q155del)) change in all affected members of family 61227. Conclusions: The results strongly suggest that the c.773G>C (p.R258P) and c.465G>T (p.(H93_Q155del)) mutations in AIPL1 cause autosomal recessive retinal degeneration in these consanguineous Pakistani families.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalMolecular vision
Volume20
StatePublished - Jan 6 2014
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

Fingerprint Dive into the research topics of 'AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration'. Together they form a unique fingerprint.

Cite this