Agrin becomes concentrated at neuroeffector junctions in developing rodent urinary bladder

J. Gingras, J. Spicer, M. Altares, Q. Zhu, G. A. Kuchel, Michael J Ferns

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The formation of somatic neuromuscular junctions in skeletal muscle is regulated by an extracellular matrix protein called agrin. Here, we have examined the expression and localization of agrin during development of the rodent urinary bladder, as a first step to examining its possible role at autonomic neuroeffector junctions in smooth muscle. We have found that agrin is expressed on the surface of developing smooth muscle cells and in the basement membrane underlying the urothelium. More importantly, agrin is progressively concentrated at parasympathetic varicosities during postnatal development and is present at virtually all junctions in mature muscle. Reverse transcription/polymerase chain reaction analysis has shown that pelvic ganglion neurons that innervate the bladder express LN/z8 agrin, whereas bladder smooth muscle expresses LN/z- agrin. Together, these results demonstrate that nerve and/or muscle agrin becomes localized at cholinergic parasympathetic varicosities in smooth muscle, where it could play a role in the maturation of the neuroeffector junction.

Original languageEnglish (US)
Pages (from-to)115-125
Number of pages11
JournalCell and Tissue Research
Volume320
Issue number1
DOIs
StatePublished - Apr 2005

Fingerprint

Neuroeffector Junction
Agrin
Muscle
Rodentia
Urinary Bladder
Smooth Muscle
Urothelium
Muscles
Neuromuscular Junction
Extracellular Matrix Proteins
Polymerase chain reaction
Transcription
Basement Membrane
Ganglia
Cholinergic Agents
Reverse Transcription
Neurons
Smooth Muscle Myocytes
Skeletal Muscle
Cells

Keywords

  • Agrin
  • Development
  • Extracellular matrix
  • Mouse
  • Parasympathetic neuroeffector junction
  • Pelvic ganglion neurons
  • Rat (Sprague Dawley)
  • Smooth muscle
  • Urinary bladder

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Agrin becomes concentrated at neuroeffector junctions in developing rodent urinary bladder. / Gingras, J.; Spicer, J.; Altares, M.; Zhu, Q.; Kuchel, G. A.; Ferns, Michael J.

In: Cell and Tissue Research, Vol. 320, No. 1, 04.2005, p. 115-125.

Research output: Contribution to journalArticle

Gingras, J. ; Spicer, J. ; Altares, M. ; Zhu, Q. ; Kuchel, G. A. ; Ferns, Michael J. / Agrin becomes concentrated at neuroeffector junctions in developing rodent urinary bladder. In: Cell and Tissue Research. 2005 ; Vol. 320, No. 1. pp. 115-125.
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