Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt, Qi Liang, Xin Y. Rairdan, Jennifer L. Moran, Haydn M. Prosser, David R. Beier, Kevin C K Lloyd, Allan Bradley, William C. Skarnes

Research output: Contribution to journalArticle

208 Scopus citations

Abstract

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Original languageEnglish (US)
Pages (from-to)493-495
Number of pages3
JournalNature Methods
Volume6
Issue number7
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

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  • Cite this

    Pettitt, S. J., Liang, Q., Rairdan, X. Y., Moran, J. L., Prosser, H. M., Beier, D. R., Lloyd, K. C. K., Bradley, A., & Skarnes, W. C. (2009). Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature Methods, 6(7), 493-495. https://doi.org/10.1038/nmeth.1342