Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
Original language | English (US) |
---|---|
Pages (from-to) | 493-495 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 6 |
Issue number | 7 |
DOIs | |
State | Published - 2009 |
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Biochemistry
- Cell Biology