Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt, Qi Liang, Xin Y. Rairdan, Jennifer L. Moran, Haydn M. Prosser, David R. Beier, Kevin C K Lloyd, Allan Bradley, William C. Skarnes

Research output: Contribution to journalArticlepeer-review

244 Scopus citations


We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Original languageEnglish (US)
Pages (from-to)493-495
Number of pages3
JournalNature Methods
Issue number7
StatePublished - 2009

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology


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