Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 493-495 |
| Number of pages | 3 |
| Journal | Nature Methods |
| Volume | 6 |
| Issue number | 7 |
| DOIs | |
| State | Published - 2009 |
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ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Biochemistry
- Cell Biology
Cite this
Agouti C57BL/6N embryonic stem cells for mouse genetic resources. / Pettitt, Stephen J.; Liang, Qi; Rairdan, Xin Y.; Moran, Jennifer L.; Prosser, Haydn M.; Beier, David R.; Lloyd, Kevin C K; Bradley, Allan; Skarnes, William C.
In: Nature Methods, Vol. 6, No. 7, 2009, p. 493-495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Agouti C57BL/6N embryonic stem cells for mouse genetic resources
AU - Pettitt, Stephen J.
AU - Liang, Qi
AU - Rairdan, Xin Y.
AU - Moran, Jennifer L.
AU - Prosser, Haydn M.
AU - Beier, David R.
AU - Lloyd, Kevin C K
AU - Bradley, Allan
AU - Skarnes, William C.
PY - 2009
Y1 - 2009
N2 - We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
AB - We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
UR - http://www.scopus.com/inward/record.url?scp=67649574240&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649574240&partnerID=8YFLogxK
U2 - 10.1038/nmeth.1342
DO - 10.1038/nmeth.1342
M3 - Article
C2 - 19525957
AN - SCOPUS:67649574240
VL - 6
SP - 493
EP - 495
JO - PLoS Medicine
JF - PLoS Medicine
SN - 1549-1277
IS - 7
ER -