Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt, Qi Liang, Xin Y. Rairdan, Jennifer L. Moran, Haydn M. Prosser, David R. Beier, Kevin C K Lloyd, Allan Bradley, William C. Skarnes

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Original languageEnglish (US)
Pages (from-to)493-495
Number of pages3
JournalNature Methods
Volume6
Issue number7
DOIs
StatePublished - 2009

Fingerprint

Stem cells
Repair
Genes
Color
Mutation
Inbred C57BL Mouse
Knockout Mice
Breeding
Cell Line
Dasyproctidae
Mouse Embryonic Stem Cells
Genetic Background

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Pettitt, S. J., Liang, Q., Rairdan, X. Y., Moran, J. L., Prosser, H. M., Beier, D. R., ... Skarnes, W. C. (2009). Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature Methods, 6(7), 493-495. https://doi.org/10.1038/nmeth.1342

Agouti C57BL/6N embryonic stem cells for mouse genetic resources. / Pettitt, Stephen J.; Liang, Qi; Rairdan, Xin Y.; Moran, Jennifer L.; Prosser, Haydn M.; Beier, David R.; Lloyd, Kevin C K; Bradley, Allan; Skarnes, William C.

In: Nature Methods, Vol. 6, No. 7, 2009, p. 493-495.

Research output: Contribution to journalArticle

Pettitt, SJ, Liang, Q, Rairdan, XY, Moran, JL, Prosser, HM, Beier, DR, Lloyd, KCK, Bradley, A & Skarnes, WC 2009, 'Agouti C57BL/6N embryonic stem cells for mouse genetic resources', Nature Methods, vol. 6, no. 7, pp. 493-495. https://doi.org/10.1038/nmeth.1342
Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR et al. Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature Methods. 2009;6(7):493-495. https://doi.org/10.1038/nmeth.1342
Pettitt, Stephen J. ; Liang, Qi ; Rairdan, Xin Y. ; Moran, Jennifer L. ; Prosser, Haydn M. ; Beier, David R. ; Lloyd, Kevin C K ; Bradley, Allan ; Skarnes, William C. / Agouti C57BL/6N embryonic stem cells for mouse genetic resources. In: Nature Methods. 2009 ; Vol. 6, No. 7. pp. 493-495.
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