Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt; Qi Liang; Xin Y. Rairdan; Jennifer L. Moran; Haydn M. Prosser; David R. Beier; Kevin C K Lloyd; Allan Bradley; William C. Skarnes

doi:10.1038/nmeth.1342

Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt, Qi Liang, Xin Y. Rairdan, Jennifer L. Moran, Haydn M. Prosser, David R. Beier, Kevin C K Lloyd, Allan Bradley, William C. Skarnes

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204 Scopus citations

Abstract

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Original language	English (US)
Pages (from-to)	493-495
Number of pages	3
Journal	Nature Methods
Volume	6
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.1342
State	Published - 2009

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ASJC Scopus subject areas

Biotechnology
Molecular Biology
Biochemistry
Cell Biology

Cite this

Pettitt, S. J., Liang, Q., Rairdan, X. Y., Moran, J. L., Prosser, H. M., Beier, D. R., Lloyd, K. C. K., Bradley, A., & Skarnes, W. C. (2009). Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature Methods, 6(7), 493-495. https://doi.org/10.1038/nmeth.1342

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10.1038/nmeth.1342