Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt; Qi Liang; Xin Y. Rairdan; Jennifer L. Moran; Haydn M. Prosser; David R. Beier; Kevin C K Lloyd; Allan Bradley; William C. Skarnes

doi:10.1038/nmeth.1342

Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J. Pettitt, Qi Liang, Xin Y. Rairdan, Jennifer L. Moran, Haydn M. Prosser, David R. Beier, Kevin C K Lloyd, Allan Bradley, William C. Skarnes

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244 Scopus citations

Abstract

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Original language	English (US)
Pages (from-to)	493-495
Number of pages	3
Journal	Nature Methods
Volume	6
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.1342
State	Published - 2009

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Biochemistry
Cell Biology

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10.1038/nmeth.1342

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title = "Agouti C57BL/6N embryonic stem cells for mouse genetic resources",

abstract = "We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.",

author = "Pettitt, {Stephen J.} and Qi Liang and Rairdan, {Xin Y.} and Moran, {Jennifer L.} and Prosser, {Haydn M.} and Beier, {David R.} and Lloyd, {Kevin C K} and Allan Bradley and Skarnes, {William C.}",

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AU - Pettitt, Stephen J.

AU - Liang, Qi

AU - Rairdan, Xin Y.

AU - Moran, Jennifer L.

AU - Prosser, Haydn M.

AU - Beier, David R.

AU - Lloyd, Kevin C K

AU - Bradley, Allan

AU - Skarnes, William C.

PY - 2009

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AB - We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

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Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Abstract

ASJC Scopus subject areas

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