Aging increases murine macrophage (M<|>) cyclooxygenase (COX-2) mRNA expression

C. V. Mura, M. G. Havek, E. Paulson, D. Hwang, S. N. Meydani

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Abstract

We previously showed that Mφ from old mice have higher PGE2 production and COX activity compared to young mice. This age-associated increase in PGE2 production contributes to decline in T cell proliferation with aging. The present study was conducted to determine the mechanism of age-associated increase in Mφ COX activity. Of the two COX isoforms identified, COX-1 (PGHS-1) is constitutively expressed in nearly all cells, the second, COX-2 (PGHS-2) is induced by a wide range of ligands. We compared levels of COX-1 and COX-2 mRNA prepared from peritoneal resident Mφ of young (5 mo) and old (22 mo) C57BL/6NIA mice. Adherent Mφ, pooled from 18-36 mice for final n=3, were stimulated with lipopolysaccharide (LPS) for 0, 2, 6, 8, 12, and 18 h. Total RNA was isolated and levels of COX-1 and COX-2 were measured by RNase protection assay. COX-1 mRNA was not detectable under these conditions. Maximum expression of COX-2 was observed at 2 h in both young and old Mφ. Mφ from old mice had higher mRNA level at 2, 4, H and 12 hrs compared to young mice. Statistically significant differences, however, were observed at 2 h (p<0.05). It is concluded that ageassociated increase in PGE2 production is in part due to higher expression of COX-2 mRNA. These findings have significant implications for ageassociated immune and inflammatory dysregulations.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Mura, C. V., Havek, M. G., Paulson, E., Hwang, D., & Meydani, S. N. (1996). Aging increases murine macrophage (M<|>) cyclooxygenase (COX-2) mRNA expression. FASEB Journal, 10(3).