Abstract
We previously showed that Mφ from old mice have higher PGE2 production and COX activity compared to young mice. This age-associated increase in PGE2 production contributes to decline in T cell proliferation with aging. The present study was conducted to determine the mechanism of age-associated increase in Mφ COX activity. Of the two COX isoforms identified, COX-1 (PGHS-1) is constitutively expressed in nearly all cells, the second, COX-2 (PGHS-2) is induced by a wide range of ligands. We compared levels of COX-1 and COX-2 mRNA prepared from peritoneal resident Mφ of young (5 mo) and old (22 mo) C57BL/6NIA mice. Adherent Mφ, pooled from 18-36 mice for final n=3, were stimulated with lipopolysaccharide (LPS) for 0, 2, 6, 8, 12, and 18 h. Total RNA was isolated and levels of COX-1 and COX-2 were measured by RNase protection assay. COX-1 mRNA was not detectable under these conditions. Maximum expression of COX-2 was observed at 2 h in both young and old Mφ. Mφ from old mice had higher mRNA level at 2, 4, H and 12 hrs compared to young mice. Statistically significant differences, however, were observed at 2 h (p<0.05). It is concluded that ageassociated increase in PGE2 production is in part due to higher expression of COX-2 mRNA. These findings have significant implications for ageassociated immune and inflammatory dysregulations.
| Original language | English (US) |
|---|---|
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 3 |
| State | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Aging increases murine macrophage (M<|>) cyclooxygenase (COX-2) mRNA expression. / Mura, C. V.; Havek, M. G.; Paulson, E.; Hwang, D.; Meydani, S. N.
In: FASEB Journal, Vol. 10, No. 3, 1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aging increases murine macrophage (M<|>) cyclooxygenase (COX-2) mRNA expression
AU - Mura, C. V.
AU - Havek, M. G.
AU - Paulson, E.
AU - Hwang, D.
AU - Meydani, S. N.
PY - 1996
Y1 - 1996
N2 - We previously showed that Mφ from old mice have higher PGE2 production and COX activity compared to young mice. This age-associated increase in PGE2 production contributes to decline in T cell proliferation with aging. The present study was conducted to determine the mechanism of age-associated increase in Mφ COX activity. Of the two COX isoforms identified, COX-1 (PGHS-1) is constitutively expressed in nearly all cells, the second, COX-2 (PGHS-2) is induced by a wide range of ligands. We compared levels of COX-1 and COX-2 mRNA prepared from peritoneal resident Mφ of young (5 mo) and old (22 mo) C57BL/6NIA mice. Adherent Mφ, pooled from 18-36 mice for final n=3, were stimulated with lipopolysaccharide (LPS) for 0, 2, 6, 8, 12, and 18 h. Total RNA was isolated and levels of COX-1 and COX-2 were measured by RNase protection assay. COX-1 mRNA was not detectable under these conditions. Maximum expression of COX-2 was observed at 2 h in both young and old Mφ. Mφ from old mice had higher mRNA level at 2, 4, H and 12 hrs compared to young mice. Statistically significant differences, however, were observed at 2 h (p<0.05). It is concluded that ageassociated increase in PGE2 production is in part due to higher expression of COX-2 mRNA. These findings have significant implications for ageassociated immune and inflammatory dysregulations.
AB - We previously showed that Mφ from old mice have higher PGE2 production and COX activity compared to young mice. This age-associated increase in PGE2 production contributes to decline in T cell proliferation with aging. The present study was conducted to determine the mechanism of age-associated increase in Mφ COX activity. Of the two COX isoforms identified, COX-1 (PGHS-1) is constitutively expressed in nearly all cells, the second, COX-2 (PGHS-2) is induced by a wide range of ligands. We compared levels of COX-1 and COX-2 mRNA prepared from peritoneal resident Mφ of young (5 mo) and old (22 mo) C57BL/6NIA mice. Adherent Mφ, pooled from 18-36 mice for final n=3, were stimulated with lipopolysaccharide (LPS) for 0, 2, 6, 8, 12, and 18 h. Total RNA was isolated and levels of COX-1 and COX-2 were measured by RNase protection assay. COX-1 mRNA was not detectable under these conditions. Maximum expression of COX-2 was observed at 2 h in both young and old Mφ. Mφ from old mice had higher mRNA level at 2, 4, H and 12 hrs compared to young mice. Statistically significant differences, however, were observed at 2 h (p<0.05). It is concluded that ageassociated increase in PGE2 production is in part due to higher expression of COX-2 mRNA. These findings have significant implications for ageassociated immune and inflammatory dysregulations.
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M3 - Article
AN - SCOPUS:33748956843
VL - 10
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 3
ER -