Age-dependent contributions of nmda receptors and l-type calcium channels to long-term depression in the piriform cortex

Vishaal Rajani, Aida Maziar, Kwun Nok Mimi Man, Johannes W. Hell, Qi Yuan

Research output: Contribution to journalArticlepeer-review


In the hippocampus, the contributions of N-methyl-D-aspartate receptors (NMDARs) and L-type calcium channels (LTCCs) to neuronal transmission and synaptic plasticity change with aging, underlying calcium dysregulation and cognitive dysfunction. However, the relative contributions of NMDARs and LTCCs in other learning encoding structures during aging are not known. The piriform cortex (PC) plays a significant role in odor associative memories, and like the hippocampus, exhibits forms of long-term synaptic plasticity. Here, we investigated the expression and contribution of NMDARs and LTCCs in long-term depression (LTD) of the PC associational fiber pathway in three cohorts of Sprague Dawley rats: neonatal (1–2 weeks), young adult (2–3 months) and aged (20–25 months). Using a combination of slice electrophysiology, Western blotting, fluorescent im-munohistochemistry and confocal imaging, we observed a shift from an NMDAR to LTCC mediation of LTD in aged rats, despite no difference in the amount of LTD expression. These changes in plasticity are related to age-dependent differential receptor expression in the PC. LTCC Cav1.2 expression relative to postsynaptic density protein 95 is increased in the associational pathway of the aged PC layer Ib. Enhanced LTCC contribution in synaptic depression in the PC may contribute to altered olfactory function and learning with aging.

Original languageEnglish (US)
Article number13551
JournalInternational journal of molecular sciences
Issue number24
StatePublished - Dec 1 2021


  • L-type calcium channel
  • Long-term depression
  • NMDA receptor
  • Piriform cortex
  • Rat

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


Dive into the research topics of 'Age-dependent contributions of nmda receptors and l-type calcium channels to long-term depression in the piriform cortex'. Together they form a unique fingerprint.

Cite this