TY - JOUR
T1 - Affinity purification of cytosolic epoxide hydrolase from human, rhesus monkey, baboon, rabbit, rat and mouse liver
AU - Silva, Marilyn H.
AU - Hammock, Bruce D.
PY - 1987
Y1 - 1987
N2 - 1. 1. An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. 2. 2. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. 3. 3. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.
AB - 1. 1. An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. 2. 2. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. 3. 3. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.
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U2 - 10.1016/0305-0491(87)90475-5
DO - 10.1016/0305-0491(87)90475-5
M3 - Article
C2 - 3608436
AN - SCOPUS:0023068610
VL - 87
SP - 95
EP - 102
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 1
ER -