Affinity purification of cytosolic epoxide hydrolase from human, rhesus monkey, baboon, rabbit, rat and mouse liver

Marilyn H. Silva, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

1. 1. An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. 2. 2. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. 3. 3. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume87
Issue number1
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Medicine(all)

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