1. 1. An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. 2. 2. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. 3. 3. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.
|Original language||English (US)|
|Number of pages||8|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|State||Published - 1987|
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