Affinity of osteogenin, an extracellular bone matrix associated protein initiating bone differentiation, for concanavalin A

Vishwas M. Paralkar, Arvind K N Nandedkar, A Hari Reddi

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Subcutaneous implantation of demineralized bone matrix results in bone differentiation. The bone inductive protein, osteogenin, was isolated recently by heparin affinity chromatography. The affinity of osteogenin for various lectins was examined to attain further purification and characterization. Osteogenin extracted from bovine bone matrix binds to concanavalin A (Con A) but not to wheat germ agglutinin or soybean lectin. The present data indicate that the bone inductive protein, osteogenin, is a glycoprotein. The use of a Con A Sepharose affinity column followed by preparative gel electrophoresis resulted in a greater than 250,000 fold purification of osteogenin.

Original languageEnglish (US)
Pages (from-to)419-424
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number2
DOIs
StatePublished - Apr 28 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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