Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time

Rona M. Silva, Donald S. Anderson, Janice Peake, Patricia C. Edwards, Esther S. Patchin, Ting Guo, Terry Gordon, Lung Chi Chen, Xiaolin Sun, Laura S. Van Winkle, Kent E Pinkerton

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Silver nanoparticle (Ag NP) production methods are being developed and refined to produce more uniform Ag NPs through chemical reactions involving silver salt solutions, solvents, and capping agents to control particle formation. These chemical reactants are often present as contaminants and/or coatings on the Ag NPs, which could alter their interactions in vivo. To determine pulmonary effects of citrate-coated Ag NPs, Sprague-Dawley rats were exposed once nose-only to aerosolized Ag NPs (20 nm [C20] or 110 nm [C110] Ag NPs) for 6 hr. Bronchoalveolar lavage fluid (BALF) and lung tissues were obtained at 1, 7, 21, and 56 days postexposure for analyses. Inhalation of Ag NPs, versus citrate buffer control, produced significant inflammatory and cytotoxic responses that were measured in BALF cells and supernatant. At day 7, total cells, protein, and lactate dehydrogenase were significantly elevated in BALF, and peak histopathology was noted after C20 or C110 exposure versus control. At day 21, BALF polymorphonuclear cells and tissue inflammation were significantly greater after C20 versus C110 exposure. By day 56, inflammation was resolved in Ag NP-exposed animals. Overall, results suggest delayed, short-lived inflammatory and cytotoxic effects following C20 or C110 inhalation and potential for greater responses following C20 exposure.

Original languageEnglish (US)
Pages (from-to)673-686
Number of pages14
JournalToxicologic Pathology
Volume44
Issue number5
DOIs
StatePublished - Jul 1 2016

Fingerprint

Bronchoalveolar Lavage Fluid
Silver
Nanoparticles
Rats
Lung
Fluids
Citric Acid
Inhalation
Exposure controls
Tissue
Inflammation
L-Lactate Dehydrogenase
Nose
Sprague Dawley Rats
Chemical reactions
Buffers
Animals
Salts
Impurities
Coatings

Keywords

  • engineered nanomaterial
  • inflammation
  • inhalation exposure
  • pulmonary toxicity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Silva, R. M., Anderson, D. S., Peake, J., Edwards, P. C., Patchin, E. S., Guo, T., ... Pinkerton, K. E. (2016). Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time. Toxicologic Pathology, 44(5), 673-686. https://doi.org/10.1177/0192623316629804

Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time. / Silva, Rona M.; Anderson, Donald S.; Peake, Janice; Edwards, Patricia C.; Patchin, Esther S.; Guo, Ting; Gordon, Terry; Chen, Lung Chi; Sun, Xiaolin; Van Winkle, Laura S.; Pinkerton, Kent E.

In: Toxicologic Pathology, Vol. 44, No. 5, 01.07.2016, p. 673-686.

Research output: Contribution to journalArticle

Silva, RM, Anderson, DS, Peake, J, Edwards, PC, Patchin, ES, Guo, T, Gordon, T, Chen, LC, Sun, X, Van Winkle, LS & Pinkerton, KE 2016, 'Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time', Toxicologic Pathology, vol. 44, no. 5, pp. 673-686. https://doi.org/10.1177/0192623316629804
Silva RM, Anderson DS, Peake J, Edwards PC, Patchin ES, Guo T et al. Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time. Toxicologic Pathology. 2016 Jul 1;44(5):673-686. https://doi.org/10.1177/0192623316629804
Silva, Rona M. ; Anderson, Donald S. ; Peake, Janice ; Edwards, Patricia C. ; Patchin, Esther S. ; Guo, Ting ; Gordon, Terry ; Chen, Lung Chi ; Sun, Xiaolin ; Van Winkle, Laura S. ; Pinkerton, Kent E. / Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time. In: Toxicologic Pathology. 2016 ; Vol. 44, No. 5. pp. 673-686.
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