Aerosol delivery of lipid: DNA complexes to lungs of rhesus monkeys

Ruth J McDonald, H. Denny Liggitt, Lisa Roche, Hung T. Nguyen, Rodney Pearlman, Otto G. Raabe, Lee B. Bussey, Cornelia M. Gorman

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Purpose. The potential use of aerosol delivery for non-viral gene therapy was tested by nebulization of lipid:DNA complexes to the lungs of rhesus monkeys. Methods. Four female rhesus monkeys were dosed with lipid:DNA formulations via aerosol inhalation, where the DNA coded for the human Cystic Fibrosis Transmembrane Conductance Regulator (hCFTR) protein. Delivery of DNA was determined in lung samples by polymerase chain reaction (PCR) by qualitative and quantitative methods. Transgene specific messenger RNA was measured by reverse transcriptase PCR (RT-PCR) and protein expression and localization were evaluated by immunohistochemistry (IHC). Results. Approximately four mg of DNA, complexed with cationic lipid 1,2-dimyristoyl- sn-glycero-3-ethylphosphatidylcholine (EDMPC) and cholesterol were delivered to the lungs of animals by air jet nebulizer. Three days after dosing, tissue samples from the lung were collected and shown to have vector specific DNA, RNA and the presence of CFTR protein. Specifically, the hCFTR protein was distributed widely, although non-uniformly, throughout airway epithelium being located on the apical surface of epithelial cells. Importantly, no adverse clinical effects were observed and the lungs showed no histological abnormalities or signs of acute inflammation. Conclusions. This study shows that lipid:DNA formulations based on EDMPC and cholesterol can be administered to primates by nebulization resulting in measurable expression of the hCFTR protein. The absence of inflammation is also encouraging and such systems may have utility for delivery of genes to the lungs for the treatment of a variety of pulmonary diseases including cystic fibrosis.

Original languageEnglish (US)
Pages (from-to)671-679
Number of pages9
JournalPharmaceutical Research
Volume15
Issue number5
DOIs
StatePublished - 1998

Keywords

  • Aerosol
  • CFTR
  • Drug delivery
  • Gene therapy
  • Lipid:DNA
  • Primates

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmaceutical Science
  • Pharmacology

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    McDonald, R. J., Liggitt, H. D., Roche, L., Nguyen, H. T., Pearlman, R., Raabe, O. G., Bussey, L. B., & Gorman, C. M. (1998). Aerosol delivery of lipid: DNA complexes to lungs of rhesus monkeys. Pharmaceutical Research, 15(5), 671-679. https://doi.org/10.1023/A:1011902532163