Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair.

F. P. Luyten, N. S. Cunningham, S. Vukicevic, V. Paralkar, U. Ripamonti, A Hari Reddi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bone matrix is a repository of growth and differentiation factors as demonstrated by the induction of local cartilage and bone formation in rats. The bone inductive activity, termed osteogenin, can be dissociatively extracted, and it was isolated by heparin affinity, hydroxyapatite and molecular sieve chromatography. Osteogenin has been purified to homogeneity from bovine bone matrix and the sequences of several tryptic peptides have been determined. The sequences were similar to portions of the amino acid sequence deduced from the cDNA clone of bone morphogenetic protein-3 (BMP-3). The carboxyl-terminal quarter of osteogenin has sequence identity to the corresponding regions of two related proteins BMP-2A and BMP-2B. The bone inductive proteins are members of the TGF-beta superfamily, by virtue of the location of the highly conserved cysteines in their carboxyl-terminal region. Osteogenin and related BMPs initiate cartilage and bone formation in vivo. The study of the mechanism of action of these proteins will add considerable new information on the molecular signals controlling endochondral bone formation. In vitro data indicate that osteogenin stimulates the expression of the osteogenic and chondrogenic phenotypes. Our results demonstrate their profound influence on proteoglycan synthesis and degradation in bovine cartilage explant cultures. High affinity specific binding sites have been identified in both MC3T3 cells and articular chondrocytes. In vivo experiments demonstrate the efficacy of primate osteogenin in restoring large calvarial defects in adult baboons, establishing a primary role for osteogenin in therapeutic initiation and promotion of osteogenesis.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalActa Orthopaedica Belgica
Volume58 Suppl 1
StatePublished - 1992
Externally publishedYes

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Bone Morphogenetic Protein 3
Bone Morphogenetic Proteins
Bone and Bones
Osteogenesis
Cartilage
Bone Matrix
TGF-beta Superfamily Proteins
Growth Differentiation Factors
Papio
Proteoglycans
Durapatite
Chondrocytes
Primates
Gel Chromatography
Cysteine
Heparin
Amino Acid Sequence
Proteins
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Surgery

Cite this

Luyten, F. P., Cunningham, N. S., Vukicevic, S., Paralkar, V., Ripamonti, U., & Reddi, A. H. (1992). Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair. Acta Orthopaedica Belgica, 58 Suppl 1, 263-267.

Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair. / Luyten, F. P.; Cunningham, N. S.; Vukicevic, S.; Paralkar, V.; Ripamonti, U.; Reddi, A Hari.

In: Acta Orthopaedica Belgica, Vol. 58 Suppl 1, 1992, p. 263-267.

Research output: Contribution to journalArticle

Luyten, FP, Cunningham, NS, Vukicevic, S, Paralkar, V, Ripamonti, U & Reddi, AH 1992, 'Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair.', Acta Orthopaedica Belgica, vol. 58 Suppl 1, pp. 263-267.
Luyten FP, Cunningham NS, Vukicevic S, Paralkar V, Ripamonti U, Reddi AH. Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair. Acta Orthopaedica Belgica. 1992;58 Suppl 1:263-267.
Luyten, F. P. ; Cunningham, N. S. ; Vukicevic, S. ; Paralkar, V. ; Ripamonti, U. ; Reddi, A Hari. / Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair. In: Acta Orthopaedica Belgica. 1992 ; Vol. 58 Suppl 1. pp. 263-267.
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