Advances in analysis of human milk oligosaccharides

L. Renee Ruhaak, Carlito B Lebrilla

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Oligosaccharides in human milk strongly influence the composition of the gut microflora of neonates. Because it is now clear that the microflora play important roles in the development of the infant immune system, human milk oligosaccharides (HMO) are studied frequently. Milk samples contain complex mixtures of HMO, usually comprising several isomeric structures that can be either linear or branched. Traditionally, HMO profiling was performed using HPLC with fluorescence or UV detection. By using porous graphitic carbon liquid chromatography MS, it is now possible to separate and identify most of the isomers, facilitating linkage-specific analysis. Matrix-assisted laser desorption ionization time-offlight analysis allows fast profiling, but does not allow isomer separation. Novel MS fragmentation techniques have facilitated structural characterization of HMO that are present at lower concentrations. These techniques now facilitate more accurate studies of HMO consumption as well as Lewis blood group determinations.

Original languageEnglish (US)
JournalAdvances in Nutrition
Volume3
Issue number3
DOIs
StatePublished - May 2012

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Human Milk
breast milk
Oligosaccharides
oligosaccharides
isomers
infant development
blood groups
Blood Group Antigens
Child Development
Complex Mixtures
intestinal microorganisms
desorption
Liquid Chromatography
ionization
liquid chromatography
lasers
linkage (genetics)
immune system
Immune System
Milk

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Advances in analysis of human milk oligosaccharides. / Ruhaak, L. Renee; Lebrilla, Carlito B.

In: Advances in Nutrition, Vol. 3, No. 3, 05.2012.

Research output: Contribution to journalArticle

Ruhaak, L. Renee ; Lebrilla, Carlito B. / Advances in analysis of human milk oligosaccharides. In: Advances in Nutrition. 2012 ; Vol. 3, No. 3.
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