Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres

Karen A. Westerman, Ashley Penvose, Zhong Yang, Paul D. Allen, Charles A. Vacanti

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The effectiveness of cell-based therapy to treat muscle disease has been hampered by difficulties in isolating, maintaining and propagating the stem cells that are needed for treatment. Here we report the isolation of muscle-derived stem cells from both young and old mice and their propagation over extended periods of time in culture as "free-floating" myospheres. Analysis of these sphere-forming cells showed that they express stem cell antigen-1 (Sca-1), β1 integrin (CD29), Thy-1 (CD90), and CD34, but did not express CD45, CD31, or myogenic markers (Pax7, Myf5, and MyoD). We found that cells derived from myospheres and then grown adherently (MDACs) behaved similar to primary myoblasts, in that these cells expressed myogenic markers and were able to easily form multinucleated myotubes. Unlike the parental myospheres but analogous to primary myoblasts, MDACs expressed Pax7, Myf5, and MyoD, indicating that the parent myosphere cells were a more primitive type of cell. In support of this we demonstrated that myospheres were also able to differentiate into adipogenic and osteogenic cells in culture, as well as being able to contribute to injured muscle in vivo. In summary, we report that primitive adult muscle stem cells can be easily isolated and sustained in culture as myospheres.

Original languageEnglish (US)
Pages (from-to)1966-1976
Number of pages11
JournalExperimental Cell Research
Volume316
Issue number12
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Adult Stem Cells
Muscle Cells
Stem Cells
Myoblasts
Muscles
Skeletal Muscle Fibers
Cell- and Tissue-Based Therapy
Integrins
Cell Culture Techniques
Antigens

Keywords

  • Multipotent
  • Myogenic
  • Myosphere
  • Non-adherent
  • Stem cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Westerman, K. A., Penvose, A., Yang, Z., Allen, P. D., & Vacanti, C. A. (2010). Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres. Experimental Cell Research, 316(12), 1966-1976. https://doi.org/10.1016/j.yexcr.2010.03.022

Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres. / Westerman, Karen A.; Penvose, Ashley; Yang, Zhong; Allen, Paul D.; Vacanti, Charles A.

In: Experimental Cell Research, Vol. 316, No. 12, 07.2010, p. 1966-1976.

Research output: Contribution to journalArticle

Westerman, KA, Penvose, A, Yang, Z, Allen, PD & Vacanti, CA 2010, 'Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres', Experimental Cell Research, vol. 316, no. 12, pp. 1966-1976. https://doi.org/10.1016/j.yexcr.2010.03.022
Westerman, Karen A. ; Penvose, Ashley ; Yang, Zhong ; Allen, Paul D. ; Vacanti, Charles A. / Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres. In: Experimental Cell Research. 2010 ; Vol. 316, No. 12. pp. 1966-1976.
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