Adsorptive effects of di-tri-octahedral smectite on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies

Jacquelin Boggs Lawler, Diana M. Hassel, Roberta J. Magnuson, Ashley E Hill, Patrick M. McCue, Josie L. Traub-Dargatz

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective - To determine the adsorptive capability of di-tri-octahedral smectite (DTOS) on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. Sample Population - 3 C perfringens exotoxins and 9 colostral samples. Procedures - Alpha, beta, and beta-2 exotoxins were individually co-incubated with serial dilutions of DTOS or bismuth subsalicylate, and the amount of toxin remaining after incubation was determined via toxin-specific ELISAs. Colostral samples from healthy mares were individually co-incubated with serial dilutions of DTOS, and colostral IgG concentrations were determined via single radial immunodiffusion assay. Results - Di-tri-octahedral smectite decreased the amount of each C perfringens exotoxin in co-incubated samples in a dose-dependent manner and was more effective than bismuth subsalicylate at reducing exotoxins in vitro. Decreases in the concentration of IgG were detected in samples of colostrum that were combined with DTOS at 1:4 through 1:16 dilutions, whereas no significant decrease was evident with DTOS at the 1:32 dilution. Conclusions and Clinical Relevance - Di-tri-octahedral smectite effectively adsorbed C perfringens exotoxins in vitro and had a dose-dependent effect on the availability of equine colostral antibodies. Results suggested that DTOS may be an appropriate adjunctive treatment in the management of neonatal clostridiosis in horses. In vivo studies are necessary to fully assess the clinical efficacy of DTOS treatment.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume69
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

exotoxins
Exotoxins
Clostridium perfringens
smectite
Horses
horses
antibodies
Antibodies
bismuth
sampling
toxins
Immunoglobulin G
Smectite
Colostrum
Immunodiffusion
dosage
in vivo studies
colostrum
mares
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Adsorptive effects of di-tri-octahedral smectite on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. / Lawler, Jacquelin Boggs; Hassel, Diana M.; Magnuson, Roberta J.; Hill, Ashley E; McCue, Patrick M.; Traub-Dargatz, Josie L.

In: American Journal of Veterinary Research, Vol. 69, No. 2, 02.2008, p. 233-239.

Research output: Contribution to journalArticle

Lawler, Jacquelin Boggs ; Hassel, Diana M. ; Magnuson, Roberta J. ; Hill, Ashley E ; McCue, Patrick M. ; Traub-Dargatz, Josie L. / Adsorptive effects of di-tri-octahedral smectite on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. In: American Journal of Veterinary Research. 2008 ; Vol. 69, No. 2. pp. 233-239.
@article{a8c3034e7dd34ef4a897c1d40cc283a4,
title = "Adsorptive effects of di-tri-octahedral smectite on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies",
abstract = "Objective - To determine the adsorptive capability of di-tri-octahedral smectite (DTOS) on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. Sample Population - 3 C perfringens exotoxins and 9 colostral samples. Procedures - Alpha, beta, and beta-2 exotoxins were individually co-incubated with serial dilutions of DTOS or bismuth subsalicylate, and the amount of toxin remaining after incubation was determined via toxin-specific ELISAs. Colostral samples from healthy mares were individually co-incubated with serial dilutions of DTOS, and colostral IgG concentrations were determined via single radial immunodiffusion assay. Results - Di-tri-octahedral smectite decreased the amount of each C perfringens exotoxin in co-incubated samples in a dose-dependent manner and was more effective than bismuth subsalicylate at reducing exotoxins in vitro. Decreases in the concentration of IgG were detected in samples of colostrum that were combined with DTOS at 1:4 through 1:16 dilutions, whereas no significant decrease was evident with DTOS at the 1:32 dilution. Conclusions and Clinical Relevance - Di-tri-octahedral smectite effectively adsorbed C perfringens exotoxins in vitro and had a dose-dependent effect on the availability of equine colostral antibodies. Results suggested that DTOS may be an appropriate adjunctive treatment in the management of neonatal clostridiosis in horses. In vivo studies are necessary to fully assess the clinical efficacy of DTOS treatment.",
author = "Lawler, {Jacquelin Boggs} and Hassel, {Diana M.} and Magnuson, {Roberta J.} and Hill, {Ashley E} and McCue, {Patrick M.} and Traub-Dargatz, {Josie L.}",
year = "2008",
month = "2",
doi = "10.2460/ajvr.69.2.233",
language = "English (US)",
volume = "69",
pages = "233--239",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "2",

}

TY - JOUR

T1 - Adsorptive effects of di-tri-octahedral smectite on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies

AU - Lawler, Jacquelin Boggs

AU - Hassel, Diana M.

AU - Magnuson, Roberta J.

AU - Hill, Ashley E

AU - McCue, Patrick M.

AU - Traub-Dargatz, Josie L.

PY - 2008/2

Y1 - 2008/2

N2 - Objective - To determine the adsorptive capability of di-tri-octahedral smectite (DTOS) on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. Sample Population - 3 C perfringens exotoxins and 9 colostral samples. Procedures - Alpha, beta, and beta-2 exotoxins were individually co-incubated with serial dilutions of DTOS or bismuth subsalicylate, and the amount of toxin remaining after incubation was determined via toxin-specific ELISAs. Colostral samples from healthy mares were individually co-incubated with serial dilutions of DTOS, and colostral IgG concentrations were determined via single radial immunodiffusion assay. Results - Di-tri-octahedral smectite decreased the amount of each C perfringens exotoxin in co-incubated samples in a dose-dependent manner and was more effective than bismuth subsalicylate at reducing exotoxins in vitro. Decreases in the concentration of IgG were detected in samples of colostrum that were combined with DTOS at 1:4 through 1:16 dilutions, whereas no significant decrease was evident with DTOS at the 1:32 dilution. Conclusions and Clinical Relevance - Di-tri-octahedral smectite effectively adsorbed C perfringens exotoxins in vitro and had a dose-dependent effect on the availability of equine colostral antibodies. Results suggested that DTOS may be an appropriate adjunctive treatment in the management of neonatal clostridiosis in horses. In vivo studies are necessary to fully assess the clinical efficacy of DTOS treatment.

AB - Objective - To determine the adsorptive capability of di-tri-octahedral smectite (DTOS) on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. Sample Population - 3 C perfringens exotoxins and 9 colostral samples. Procedures - Alpha, beta, and beta-2 exotoxins were individually co-incubated with serial dilutions of DTOS or bismuth subsalicylate, and the amount of toxin remaining after incubation was determined via toxin-specific ELISAs. Colostral samples from healthy mares were individually co-incubated with serial dilutions of DTOS, and colostral IgG concentrations were determined via single radial immunodiffusion assay. Results - Di-tri-octahedral smectite decreased the amount of each C perfringens exotoxin in co-incubated samples in a dose-dependent manner and was more effective than bismuth subsalicylate at reducing exotoxins in vitro. Decreases in the concentration of IgG were detected in samples of colostrum that were combined with DTOS at 1:4 through 1:16 dilutions, whereas no significant decrease was evident with DTOS at the 1:32 dilution. Conclusions and Clinical Relevance - Di-tri-octahedral smectite effectively adsorbed C perfringens exotoxins in vitro and had a dose-dependent effect on the availability of equine colostral antibodies. Results suggested that DTOS may be an appropriate adjunctive treatment in the management of neonatal clostridiosis in horses. In vivo studies are necessary to fully assess the clinical efficacy of DTOS treatment.

UR - http://www.scopus.com/inward/record.url?scp=39549096212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39549096212&partnerID=8YFLogxK

U2 - 10.2460/ajvr.69.2.233

DO - 10.2460/ajvr.69.2.233

M3 - Article

C2 - 18241021

AN - SCOPUS:39549096212

VL - 69

SP - 233

EP - 239

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 2

ER -