Adrenocortical cytochrome b5 expression during fetal development of the rhesus macaque

Samantha Mapes, Alice F Tarantal, C. Richard Parker, Francisco M. Moran, Janice M. Bahr, Leah Pyter, Alan J Conley

Research output: Contribution to journalArticle

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Abstract

The developmental expressions of cytochrome b5 (b5), 17α-hydroxylase/17,20-lyase cytochrome P450 (P450c17), and 3β-hydroxysteroid dehydrogenase were examined in primate fetal adrenals by immunocytochemistry from 50-160 d gestation. The expression of b5 was evident at 50 d in the developing fetal zone (FZ), but decreased markedly through midgestation, then increased again from 150 d to term. Similar changes in the temporal expression was observed for P450c17. Whereas P450c17 was induced largely in the transitional zone (TZ; outer-most FZ), b5 expression was strongest in FZ cells further from the capsule, although overlap between these regions involved a narrow band of cells beneath the TZ that may represent the developing zona reticularis. Thus, the induction of b5 in the FZ and of P450c17 in the TZ of the fetal adrenal late in gestation coincided temporally with the prepartum rise in dehydroepiandrosterone previously reported. These data are consistent with the proposed role of b5 in supporting 17,20-lyase activity of P450c17. However, the lack of cytochrome b5 and P450c17 expression in the FZ of the developing macaque adrenal cortex for much of the second and third trimesters distinguishes it from the mature zona reticularis seen in adult animals.

Original languageEnglish (US)
Pages (from-to)1451-1458
Number of pages8
JournalEndocrinology
Volume143
Issue number4
DOIs
StatePublished - 2002

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Zona Reticularis
Steroid 17-alpha-Hydroxylase
Cytochromes b5
Fetal Development
Macaca mulatta
3-Hydroxysteroid Dehydrogenases
Pregnancy
Dehydroepiandrosterone
Adrenal Cortex
Third Pregnancy Trimester
Macaca
Second Pregnancy Trimester
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Primates
Capsules
Immunohistochemistry

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Adrenocortical cytochrome b5 expression during fetal development of the rhesus macaque. / Mapes, Samantha; Tarantal, Alice F; Richard Parker, C.; Moran, Francisco M.; Bahr, Janice M.; Pyter, Leah; Conley, Alan J.

In: Endocrinology, Vol. 143, No. 4, 2002, p. 1451-1458.

Research output: Contribution to journalArticle

Mapes, Samantha ; Tarantal, Alice F ; Richard Parker, C. ; Moran, Francisco M. ; Bahr, Janice M. ; Pyter, Leah ; Conley, Alan J. / Adrenocortical cytochrome b5 expression during fetal development of the rhesus macaque. In: Endocrinology. 2002 ; Vol. 143, No. 4. pp. 1451-1458.
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